Hypercyst mutants in Rhodospirillum centenum identify regulatory loci involved in cyst cell differentiation
Hypercyst mutants in Rhodospirillum centenum identify regulatory loci involved in cyst cell differentiation
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DOI：
10.1128/jb.186.17.5834-5841.2004
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发表时间：
2004-09-01
影响因子：
3.2
通讯作者：
Bauer, CE
中科院分区：
文献类型：
--
作者：
Berleman, JE;Hasselbring, BM;Bauer, CE
百年红螺菌是一种紫色的光合作用细菌，在缺乏营养的情况下会形成休眠的包囊细胞。在这项研究中，我们证明查尔酮合成酶基因(CHSA)的表达受发育调节，在诱导包囊发育周期时，CHSA的表达增加了86倍。对Mini-Tn5诱导的CHSA：：LacZ表达升高的突变体的筛选导致分离出一组伴随着孢子发育周期的结构性诱导而表现出CHSA基因表达增加的百合根霉突变体。这些“超级囊泡”突变体已经失去了调节囊泡细胞形成以回应营养供应的能力。序列分析表明，微小的TN5干扰基因编码多种因子，包括代谢酶和一大组潜在的调节因子，包括四个与组氨酸感受器激酶同源的基因产物和三个与反应调节同源的基因产物。一些被破坏的基因也与ChE样信号转导组件具有序列相似性。
Rhodospirillum centenum is a purple photosynthetic bacterium that forms resting cyst cells when starved for nutrients. In this study, we demonstrate that chalcone synthase gene (chsA) expression is developmentally regulated, with expression of chsA increasing up to 86-fold upon induction of the cyst developmental cycle. Screening for mini-Tn5-induced mutants that exhibit elevated chsA::lacZ expression has led to the isolation of a set of R. centenum mutants that display increased chsA gene expression concomitant with constitutive induction of the cyst developmental cycle. These "hypercyst" mutants have lost the ability to regulate cyst cell formation in response to nutrient availability. Sequence analysis indicates that the mini-Tn5-disrupted genes code for a variety of factors, including metabolic enzymes and a large set of potential regulatory factors, including four gene products with homology to histidine sensor kinases and three with homology to response regulators. Several of the disrupted genes also have sequence similarity to che-like signal transduction components.