A Pillar and Perfusion Plate Platform for Robust Human Organoid Culture and Analysis
A Pillar and Perfusion Plate Platform for Robust Human Organoid Culture and Analysis
复制标题
用于稳健人类类器官培养和分析的支柱和灌注板平台
DOI：
10.1101/2023.03.11.532210
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Lee MY.
中科院分区：
文献类型：
--
作者：
Kang SY;Kimura M;Shrestha S;Lewis P;Lee S;Cai Y;Joshi P;Acharya P;Liu J;Yang Y;Sanchez JG;Ayyagari S;Alsberg E;Wells JM;Takebe T;Lee MY.
人类类器官通过提供与体内相似的多细胞结构和功能，有可能彻底改变体外疾病建模。然而，由于繁琐的类器官分化过程以及规模化和质量控制方面的困难，这种创新和不断发展的技术在实现化合物的高通量筛选（HTS）方面仍然存在分析通量和可重复性的问题。由于缺乏与相对较大的类器官兼容的易于使用的流体系统，将类器官用于HTS进一步受到挑战。在这里，这些挑战是通过工程“微阵列三维（3D）生物打印”技术和相关的柱和灌注板来克服的，用于人类类器官的培养和分析。高精度，高通量干细胞打印和封装技术在柱板上进行了演示，柱板与一个互补的深孔板和灌注孔板相结合，用于静态和动态类器官培养。水凝胶中的生物打印细胞和球体分化为肝脏和肠道类器官，用于原位功能测定。柱/灌注板与标准384孔板和HTS设备兼容，因此可以很容易地用于当前的药物发现工作。
Human organoids have the potential to revolutionize in vitro disease modeling by providing multicellular architecture and function that are similar to those in vivo. This innovative and evolving technology, however, still suffers from assay throughput and reproducibility to enable high‐throughput screening (HTS) of compounds due to cumbersome organoid differentiation processes and difficulty in scale‐up and quality control. Using organoids for HTS is further challenged by the lack of easy‐to‐use fluidic systems that are compatible with relatively large organoids. Here, these challenges are overcome by engineering “microarray three‐dimensional (3D) bioprinting” technology and associated pillar and perfusion plates for human organoid culture and analysis. High‐precision, high‐throughput stem cell printing, and encapsulation techniques are demonstrated on a pillar plate, which is coupled with a complementary deep well plate and a perfusion well plate for static and dynamic organoid culture. Bioprinted cells and spheroids in hydrogels are differentiated into liver and intestine organoids for in situ functional assays. The pillar/perfusion plates are compatible with standard 384‐well plates and HTS equipment, and thus may be easily adopted in current drug discovery efforts.
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影响因子：
23.9
作者：
Czerniecki SM;Cruz NM;Harder JL;Menon R;Annis J;Otto EA;Gulieva RE;Islas LV;Kim YK;Tran LM;Martins TJ;Pippin JW;Fu H;Kretzler M;Shankland SJ;Himmelfarb J;Moon RT;Paragas N;Freedman BS
通讯作者：
Freedman BS
通过诱导多能干细胞衍生的器官芽移植生成人类肝脏组织。
DOI：
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发表时间：
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期刊：
影响因子：
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作者：
武部貴則;小林眞司;矢吹雄一郎;鈴木啓;水野満;安村和正;広富浩一;鄭允文;前川二郎;谷口英樹;Takebe T;Enomura M;Zheng Y;Zhang R;Sekine K;Mizuno M;武部貴則;武部貴則;武部貴則;谷口英樹;Takanori Takebe;Takanori Takebe
通讯作者：
Takanori Takebe
DOI：
10.1007/978-1-4939-6949-4_12
发表时间：
2017-01-01
期刊：
ORGAN REGENERATION: 3D STEM CELL CULTURE & MANIPULATION
影响因子：
--
作者：
Munera, Jorge O.;Wells, James M.
通讯作者：
Wells, James M.
影响因子：
7.7
作者：
Achberger, Kevin;Probst, Christopher;Loskill, Peter
通讯作者：
Loskill, Peter
影响因子：
29.4
作者：
Chalasani N;Björnsson E
通讯作者：
Björnsson E