The effect of acetoacetate on plasma insulin concentration.
The effect of acetoacetate on plasma insulin concentration.
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乙酰乙酸对血浆胰岛素浓度的影响。
DOI：
10.1042/bj1250541
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发表时间：
1971
期刊：
影响因子：
--
通讯作者：
H. Krebs
中科院分区：
文献类型：
--
作者：
R. Hawkins;K. Alberti;C. Houghton;D. Williamson;H. Krebs
1.乙酰乙酸钠注入下腔静脉喂养大鼠，48小时饥饿大鼠，和喂养链脲佐菌素糖尿病大鼠用胰岛素治疗。从股动脉导管获得动脉血。2.乙酰乙酸酯输注引起进食大鼠的血糖浓度在1h内从6.16降至5.11mm，而饥饿或进食糖尿病大鼠没有发生变化。3.血浆游离脂肪酸在10分钟内从0.82降至0.64毫当量/升。l，1.16 - 0.79毫当量/ l，0.83 - 0.65mequiv./ l在糖尿病大鼠中。4.在10分钟时，进食未麻醉大鼠的血药浓度从20 μ单位/ml上升到49.9 μ单位/ml，饥饿大鼠的血药浓度从6.4 μ单位/ml上升到18.5 μ单位/ml。糖尿病大鼠的胰岛素浓度无变化。5.戊巴比妥麻醉后，大鼠血浆胰岛素浓度在10分钟内由30 μ units/ml增加到101 μ units/ml。6.由于血浆胰岛素增加，预期进食大鼠的血糖浓度下降，以及进食和饥饿大鼠的游离脂肪酸减少。7.糖尿病大鼠游离脂肪酸浓度的下降可能是由于酮体对脂肪组织的直接影响。对游离脂肪酸的类似作用也可在正常进食或饥饿大鼠中起作用。
1. Sodium acetoacetate was infused into the inferior vena cava of fed rats, 48h-starved rats, and fed streptozotocin-diabetic rats treated with insulin. Arterial blood was obtained from a femoral artery catheter. 2. Acetoacetate infusion caused a fall in blood glucose concentration in fed rats from 6.16 to 5.11mm in 1h, whereas no change occurred in starved or fed-diabetic rats. 3. Plasma free fatty acids decreased within 10min, from 0.82 to 0.64mequiv./l in fed rats, 1.16 to 0.79mequiv./l in starved rats and 0.83 to 0.65mequiv./l in fed-diabetic rats. 4. At 10min the plasma concentration rose from 20 to 49.9muunits/ml in fed unanaesthetized rats and from 6.4 to 18.5muunits/ml in starved rats. There was no change in insulin concentration in the diabetic rats. 5. Nembutal-anaesthetized fed rats had a more marked increase in plasma insulin concentration, from 30 to 101muunits/ml within 10min. 6. A fall in blood glucose concentration in fed rats and a decrease in free fatty acids in both fed and starved rats is to be expected as a consequence of the increase in plasma insulin. 7. The fall in the concentration of free fatty acids in diabetic rats may be due to a direct effect of ketone bodies on adipose tissue. A similar effect on free fatty acids could also be operative in normal fed or starved rats.