Biotransformation of phenolics with laccase containing bacterial spores
Biotransformation of phenolics with laccase containing bacterial spores
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DOI：
10.1007/s10311-005-0006-1
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发表时间：
2005-11-01
影响因子：
15.7
通讯作者：
Guebitz, G. M.
中科院分区：
文献类型：
--
作者：
Held, C.;Kandelbauer, A.;Guebitz, G. M.
用真菌漆酶处理含酚废水如纺织染料通常限于酸性至中性pH范围和中等温度。在这里，我们证明了第一次，孢子结合的漆酶是稳定的，在高温和pH值可以用于酚类染料脱色。将含芽孢杆菌SF芽孢的漆酶固定在氧化铝颗粒上。固定化和游离孢子都能够在90分钟的孵育时间内完全脱色常见的纺织染料媒介黑9，媒介棕96/媒介棕15，和酸性蓝74，脱色溶液成功地用于再染色。
Treatment of effluents containing phenols such as textile dyes with fungal laccases is usually limited to the acid to neutral pH range and moderate temperatures. Here we demonstrate for the first time that spore-bound laccases which are stable at high temperatures and pH values can be used for phenolic dye decolorisation. Laccase containing spores from Bacillus SF were immobilized on alumina pellets. Both immobilized and free spores were able to completely decolorize the common textile dyes Mordant Black 9, Mordant Brown 96/Mordant Brown 15, and Acid Blue 74 within 90 min of incubation time and decolorized solutions were successfully used in re-dyeing.