Non-structural flagella genes affecting both polar and lateral flagella-mediated motility in Aeromonas hydrophila
Non-structural flagella genes affecting both polar and lateral flagella-mediated motility in Aeromonas hydrophila
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DOI：
10.1099/mic.0.2006/000687-0
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发表时间：
2007-04-01
期刊：
影响因子：
2.8
通讯作者：
Tomas, Juan M.
中科院分区：
文献类型：
--
作者：
Canals, Rocio;Vilches, Silvia;Tomas, Juan M.
分离到一株不能产生极鞭毛和侧鞭毛的嗜水气单胞菌AH-3 miniTn 5突变株，该突变株将转座子插入到一个基因中，该基因编码的蛋白质是空肠弯曲菌运动辅助因子（Maf）蛋白的直向同源物。除了该基因外，在该基因簇中还发现了与极鞭毛2区基因相邻的其他几个相关基因。A. Escherichia AH-3 maf-2、neuB样、flmD或neuA样基因导致非运动细胞，其由于缺少极鞭毛和侧鞭毛而不能游泳或群集。然而，极性和侧鞭毛蛋白都存在，但未糖基化。虽然A.大肠杆菌AH-3或Caerospora Sch 3 N基因在核苷酸水平上不相互杂交，基因产物能够完全互补任一细菌中的突变。此外，C. jejuni鞭毛糖基化相关基因（Cj 1293、-1294和-1317）完全能够与A.在相应的基因（flmA，flmB和neuB样）中的突变体。结果表明，maf-2、neuB-like、flmD和neuA-like基因参与了气单胞菌极鞭毛和侧鞭毛的糖基化。
An Aeromonas hydrophila AH-3 miniTn5 mutant unable to produce polar and lateral flagella was isolated, in which the transposon was inserted into a gene whose encoded protein was an orthologue of the Campylobacter jejuni motility accessory factor (Maf) protein. In addition to this gene, several other related genes were found in this cluster that was adjacent to the region 2 genes of the polar flagellum. Mutation of the A. hydrophila AH-3 maf-2, neuB-like, flmD or neuA-like genes resulted in non-motile cells that were unable to swim or swarm due to the absence of both polar and lateral flagella. However, both polar and lateral flagellins were present but were unglycosylated. Although the A. hydrophila AH-3 or Aeromonas caviae Sch3N genes did not hybridize with each other at the nucleotide level, the gene products were able to fully complement the mutations in either bacterium. Furthermore, well-characterized C. jejuni genes involved in flagella glycosylation (Cj 1293, -1294 and -1317) were fully able to complement A. hydrophila mutants in the corresponding genes (flmA, flmB and neuB-like). It was concluded that the maf-2, neuB-like, flmD and neuA-like genes are involved in the glycosylation of both the polar and the lateral flagella in Aeromonas strains.