AMINO-ACID ALTERATIONS IN THE BETA-TUBULIN GENE OF NEUROSPORA-CRASSA THAT CONFER RESISTANCE TO CARBENDAZIM AND DIETHOFENCARB
AMINO-ACID ALTERATIONS IN THE BETA-TUBULIN GENE OF NEUROSPORA-CRASSA THAT CONFER RESISTANCE TO CARBENDAZIM AND DIETHOFENCARB
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DOI：
10.1007/bf00351780
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发表时间：
1994-05-01
期刊：
影响因子：
2.5
通讯作者：
YAMAGUCHI, I
中科院分区：
文献类型：
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作者：
FUJIMURA, M;KAMAKURA, T;YAMAGUCHI, I
我们之前已经表明，粗糙脉孢菌的多菌灵 (MBC) 抗性 F914 菌株对敌虫威的敏感性增加是由 β-微管蛋白中的单个氨基酸变化（198）Glu 变为 Gly 引起的。从菌株 F914 中分离出三种对乙虫威具有抗性且对 MBC 具有抗性的突变体。它们在β-微管蛋白基因中含有单碱基对取代突变。 β-微管蛋白中的氨基酸变化，即 (250)Leu 的 Phe、(165)Ala 的 Val 和 (237)Thr 的 Ala 的变化分别导致突变株 FR511、FR513 和 FR421 中乙虫威耐药。这些突变体中β-微管蛋白198位的氨基酸是Gly，与菌株F914中的相同。通过定点诱变构建具有(198)Glu的β-微管蛋白基因。源自 FR511 和 FR421 的改变的 β-微管蛋白基因使野生型菌株对 MBC 产生抗性，表明 β-微管蛋白中的 (250)Phe 和 (237)Ala 不仅负责乙虫威的抗性，而且还负责对 MBC 的抗性。
We have previously shown that increased sensitivity to diethofencarb in the carbendazim(MBC)-resistant F914 strain of Neurospora crassa is caused by a single amino-acid change in beta-tubulin, (198)Glu to Gly. Three diethofencarb-resistant mutants that are also resistant to MBC were isolated from strain F914. They contained single base-pair-substitution mutations in the beta-tubulin gene. The amino acid changes in beta-tubulin, Phe from (250)Leu, Val from (165)Ala, and Ala from (237)Thr, were responsible for diethofencarb-resistance in the mutant strains FR511, FR513, and FR421, respectively. The amino acid at position 198 of beta-tubulin in these mutants was Gly, which is the same as in strain F914. beta-tubulin genes with (198)Glu were constructed by site-directed mutagenesis. The altered beta-tubulin genes derived from FR511 and FR421 transformed the wild-type strain to resistance to MBC, indicating that (250)Phe and (237)Ala in beta-tubulin are responsible for resistance not only to diethofencarb but also to MBC.