Orthotopic liver transplantation in the mouse.
Orthotopic liver transplantation in the mouse.
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DOI：
10.1097/00007890-199109000-00039
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发表时间：
1991-09
期刊：
影响因子：
6.2
通讯作者：
S. Qian;J. Fung;A. Demetris;S. Ildstad;T. Starzl
中科院分区：
文献类型：
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作者：
S. Qian;J. Fung;A. Demetris;S. Ildstad;T. Starzl
小鼠的心脏和肾脏移植已经得到了很好的建立（1，2），但原位肝移植尚未报道。虽然老鼠的大小是大鼠的十分之一，但使用老鼠进行免疫学研究有许多优点。小鼠的基因组比大鼠或任何其他哺乳动物的基因组都更彻底。小鼠H-2系统也与人类HLA系统有着惊人的相似之处。此外，有许多遗传上确定的近交系和丰富的单克隆抗体可用于小鼠研究。大鼠原位肝移植的一个关键进展（3）是齐默尔曼等人（4）以及Kamada和Caine（5）引入的袖套技术，用于治疗某些静脉血管闭塞。该方法缩短了门静脉阻断时间，提高了存活率。我们已经将这一原则应用于小鼠原位肝移植。在我们的初步研究中，在获得6个长期存活者之前，进行了40多个同基因小鼠肝脏置换。这里报告的经验是接下来的48次尝试，其中手术成功率为83%。将来自杰克逊实验室（Bar Harbor，ME）的10-12周龄（25-32 g）雄性近交系同基因小鼠用作大小匹配的供体和受体，并在甲氧氟烷（2.2-二氯-1，1-二氟乙基甲基醚）麻醉下进行。使用的菌株为B6 AF 1（27对）、C57 BL/6（11对）和BALB/c（10对）。采用清洁但非无菌的手术技术，所有手术均在4-6.4倍放大的手术显微镜下进行。
Heart and kidney transplantation in the mouse has been well established (1, 2), but orthotopic liver transplantation has not been reported. Although the mouse is one-tenth the size of the rat, there are many advantages to using the mouse for immunologic research. The mouse genome has been more thoroughly characterized than the rat or any other species of mammal. The mouse H-2 system also bears a striking resemblance to the human HLA system. In addition, there are numerous genetically defined inbred strains and wealth of monoclonal antibodies that are commercially available for mouse investigations. A key development in rat orthotopic liver transplantation (3) was the introduction by Zimmerman et al. (4) and Kamada and Caine (5), of the cuff technique instead of suture for some of the venous vascular anastomoses. This method shortened the clamping time of the portal vein and increased survival. We have applied this principle to mouse orthotopic liver transplantation. In our pilot studies, more than 40 syngeneic mouse liver replacements were performed before 6 long-term survivors were obtained. The experience reported here is with the next 48 attempts, in which the surgical success rate was 83%. Male inbred syngeneic mice 10–12 weeks old (25–32 g), from Jackson Laboratory, Bar Harbor, ME, were used as size-matched donors and recipients under methoxyflurane (2.2-dichloro-1,1-difluoroethyl methyl ether) anesthesia. The strains used were B6AF1 (27 pairs), C57BL/6 (11 pairs), and BALB/c (10 pairs). Clean but not sterile operative technique was used, and all procedures were performed under the operating microscope with 4–6.4× magnification.