Efficient reversed-phase purification of a hydrophobic reaction product following Mitsunobu-mediated glycosylation.
Efficient reversed-phase purification of a hydrophobic reaction product following Mitsunobu-mediated glycosylation.
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Mitsunobu 介导的糖基化后疏水反应产物的高效反相纯化。
DOI：
10.1016/s0021-9673(01)01390-5
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
Zembower,DavidE
中科院分区：
文献类型：
--
作者：
Iyer,ManiS;Palomo,Martin;Schilling,KevinM;Xie,Yongping;Formanski,Leo;Zembower,DavidE
Mitsunobu 反应用于将四-O-苄基-d-吡喃葡萄糖连接到单吲哚基马来酰亚胺上，为吲哚并咔唑拓扑异构酶 I 毒物的全合成提供了关键中间体。使用正相硅胶色谱法，糖基化产物的纯化通常需要多个柱，导致回收率较差。反相色谱法被成功地用于快速且高产率地纯化这种高度疏水性的材料。
The Mitsunobu reaction was used to attach tetra-O-benzyl-d-glucopyranose to a monoindolylmaleimide, providing a key intermediate in the total synthesis of indolocarbazole topoisomerase I poisons. Using normal-phase silica gel chromatography, purification of the glycosylated product normally required multiple columns, resulting in poor recovered yields. Reversed-phase chromatography was used successfully to purify this highly hydrophobic material, rapidly and in high yield.