Expression of Cholecystokinin, Gastrin, and Their Receptors in the Mouse Cornea
Expression of Cholecystokinin, Gastrin, and Their Receptors in the Mouse Cornea
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DOI：
10.1167/iovs.13-12068
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发表时间：
2014-03-01
影响因子：
4.4
通讯作者：
Belmonte, Carlos
中科院分区：
文献类型：
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作者：
Gonzalez-Coto, Ana F.;Alonso-Ron, Carlos;Belmonte, Carlos
目的。胆囊素(CCK)是三叉神经节神经元中发现的一种神经肽。胃泌素(GAST)是一种从未在角膜中发现的相关多肽。这两种胃肠肽在角膜和角膜感觉神经元中的存在和作用仍有待确定。方法采用RT-PCR方法检测小鼠角膜上皮、三叉神经节和原代培养的角膜上皮细胞、三叉神经节和原代培养的角膜上皮细胞中CCK、GAST、CCK1R和CCK2R的表达。用免疫荧光显微镜对这些多肽及其受体在角膜、培养的角膜上皮细胞和角膜神经中进行定位，并用Fast Blue逆行标记鉴定其在角膜三叉神经节神经元胞体中的表达。结果：小鼠角膜上皮细胞在原位和在细胞培养中均表达CCK和GAST。仅在角膜上皮细胞中发现CCK2R受体。此外，小鼠角膜传入感觉神经元表达CCK、GAST及其受体。结论CCK、GAST及其受体在小鼠角膜上皮细胞和为角膜提供感觉神经支配的三叉神经节神经元中的存在，开启了这些神经肽参与角膜神经源性炎症和角膜损伤后修复/重塑过程的调控的可能性。
PURPOSE. Cholecystokinin (CCK) is a neuropeptide that has been identified in trigeminal ganglion neurons. Gastrin (GAST) is a related peptide never explored in the cornea. The presence and role of both gastrointestinal peptides in the cornea and corneal sensory neurons remain to be established. We explored here in mice whether CCK, GAST, and their receptors CCK1R and CCK2R are expressed in the corneal epithelium and trigeminal ganglion neurons innervating the cornea.METHODS. We used RT-PCR analysis to detect mRNAs of CCK, GAST, CCK1R, and CCK2R in mouse cornea epithelium, trigeminal ganglia, and primary cultured corneal epithelial cells. Immunofluorescence microscopy was used to localize these peptides and their receptors in the cornea, cultured corneal epithelial cells, and corneal nerves, as well as in the cell bodies of corneal trigeminal ganglion neurons identified by retrograde labeling with Fast Blue.RESULTS. Mouse corneal epithelial cells in the cornea in situ and in cell cultures expressed CCK and GAST. Only the receptor CCK2R was found in the corneal epithelium. In addition, mouse corneal afferent sensory neurons expressed CCK and GAST, and the CCK1R receptors.CONCLUSIONS. The presence of CCK, GAST, and their receptors in the mouse corneal epithelium, and in trigeminal ganglion neurons supplying sensory innervation to the cornea, opens the possibility that these neuropeptides are involved in corneal neurogenic inflammation and in the modulation of repairing/remodeling processes following corneal injury.