Topical acidic cream prevents the development of atopic dermatitis- and asthma-like lesions in murine model
Topical acidic cream prevents the development of atopic dermatitis- and asthma-like lesions in murine model
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DOI：
10.1111/exd.12525
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发表时间：
2014-10-01
影响因子：
3.6
通讯作者：
Choi, Eung Ho
中科院分区：
文献类型：
--
作者：
Lee, Hae-Jin;Yoon, Na Young;Choi, Eung Ho
特应性皮炎（AD）的长期或反复皮肤屏障损伤是特应性进展的第一步，最终发展为呼吸道过敏。角质层（SC）中酸性pH的维持是正常皮肤屏障功能的重要因素。我们进行了这项研究，以确定恶唑酮（Ox）诱导的AD小鼠模型是否可以通过局部应用和鼻吸入屋尘螨，屋尘螨（Dermatofagoides pteronyssinus）（Dp）（这是一种新的特应性行军动物模型）来发展气道炎症，以及通过重复应用酸性乳膏形成的酸性SC环境是否可以中断这种特应性行军。在用Ox和Dp重复处理以制备特应性行军鼠模型期间，每天两次施用通过与媒介物混合的柠檬酸和氢氧化钠调节的酸性乳膏（pH 2.8）和中性乳膏（pH 7.4）。用Ox和Dp重复处理无毛小鼠诱导AD样皮肤病变，随后是呼吸道过敏，将其定义为特应性行军模型。酸性乳膏可抑制呼吸道过敏性炎症和AD样皮肤病变的发生。这些结果表明，一种新的特应性行军动物模型，可以通过反复局部和鼻腔处理的屋尘螨对奥克斯诱导的AD小鼠和SC的酸化可能是一种新的干预方法，以阻止特应性行军。
Long-standing or repeated skin barrier damage followed by atopic dermatitis (AD) is the initial step of the atopic march that eventually progresses to respiratory allergies. Maintenance of an acidic pH in the stratum corneum (SC) is an important factor for normal skin barrier function. We performed this study to determine whether an oxazolone (Ox)-induced AD murine model can develop airway inflammation by topical application and nasal inhalation of a house dust mite, Dermatofagoides pteronyssinus (Dp), which is a novel atopic march animal model', and whether an acidic SC environment, made by repeated application of acidic cream, can interrupt this atopic march. During repeated treatment with Ox and Dp to make an atopic march murine model, acidic cream (pH 2.8) and neutral cream (pH 7.4) adjusted by citric acid and sodium hydroxide mixed with vehicle were applied twice daily. Repeated treatment with Ox and Dp to hairless mice induced AD-like skin lesions followed by respiratory allergy, defining it as an atopic march model. Acidic cream inhibited the occurrence of respiratory allergic inflammation as well as AD-like skin lesions. These results indicate that a novel atopic march animal model can be developed by repeated topical and nasal treatments with house dust mite on Ox-induced AD mice and that the acidification of SC could be a novel intervention method to block the atopic march.