Balancing crosstalk between 20-hydroxyecdysone-induced autophagy and caspase activity in the fat body during Drosophila larval-prepupal transition
Balancing crosstalk between 20-hydroxyecdysone-induced autophagy and caspase activity in the fat body during Drosophila larval-prepupal transition
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DOI：
10.1016/j.ibmb.2013.09.001
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发表时间：
2013-11-01
影响因子：
3.8
通讯作者：
Li, Sheng
中科院分区：
文献类型：
--
作者：
Liu, Hanhan;Jia, Qiangqiang;Li, Sheng
在果蝇、黑腹果蝇中，自噬和caspase活性在变态期间的唾液腺和卵子发生期间的共同调控层次中平行发挥作用。在重塑的脂肪体中，自噬和caspase活性都逐渐增加，它们是在幼虫-预蛹转变过程中由蜕皮激素(20-羟基蜕皮酮，20E)的脉冲诱导的。抑制自噬和/或重塑脂肪体中的caspase活性会导致25%-40%的蛹死亡，这取决于不同的基因类型。有趣的是，在自噬和caspase活性之间发生了一个平衡的串扰：抑制自噬诱导caspase活性，抑制caspase诱导自噬。果蝇脂肪体重塑为理解自噬和caspase活性之间平衡串扰的分子机制提供了一个体内模型，自噬和caspase活性相互对立，由共同的刺激20E诱导，其中一条途径的阻断加强了另一条途径。(C)2013爱思唯尔有限公司。保留所有权利。
In the fruitfly, Drosophila melanogaster, autophagy and caspase activity function in parallel in the salivary gland during metamorphosis and in a common regulatory hierarchy during oogenesis. Both autophagy and caspase activity progressively increase in the remodeling fat body, and they are induced by a pulse of the molting hormone (20-hydroxyecdysone, 20E) during the larval-prepupal transition. Inhibition of autophagy and/or caspase activity in the remodeling fat body results in 25-40% pupal lethality, depending on the genotypes. Interestingly, a balancing crosstalk occurs between autophagy and caspase activity in this tissue: the inhibition of autophagy induces caspase activity and the inhibition of caspases induces autophagy. The Drosophila remodeling fat body provides an in vivo model for understanding the molecular mechanism of the balancing crosstalk between autophagy and caspase activity, which oppose with each other and are induced by the common stimulus 20E, and blockage of either path reinforces the other path. (C) 2013 Elsevier Ltd. All rights reserved.