Mucosal macrophage inflammatory protein-1α activity in Helicobacter pylori infection
Mucosal macrophage inflammatory protein-1α activity in Helicobacter pylori infection
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DOI：
10.1046/j.1440-1746.1999.01810.x
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发表时间：
1999-01-01
影响因子：
4.1
通讯作者：
Kaneko, H
中科院分区：
文献类型：
--
作者：
Kusugami, K;Ando, T;Kaneko, H
粘液趋化因子被认为在幽门螺杆菌相关性胃炎的发病机制中起重要作用。本研究的目的是检测巨噬细胞炎性蛋白-1 α（MIP-1 α）在器官培养中的水平、MIP-1 α mRNA的表达以及MIP-1 α的细胞来源。pylori阳性和阴性患者。采用酶联免疫吸附试验测定粘膜组织器官培养物和分离粘膜细胞的细胞培养物中MIP-1 α的水平。分别采用逆转录聚合酶链反应（RT-PCR）和免疫荧光双标显微镜检测MIP-1 α mRNA和蛋白的表达。从H.幽门螺杆菌阳性患者在器官培养中MIP-1 α活性值显著高于幽门螺杆菌阳性患者，固有层中CD 68（+）巨噬细胞、髓过氧化物酶（+）中性粒细胞和单核细胞数量增加。幽门阴性患者。RT-PCR检测MIP-1 α mRNA在50%以上的H. pylori感染，而不是在那些没有感染。在细胞培养物中，巨噬细胞部分含有实质上更高的量的MIP-1 α在每个细胞的基础上比淋巴细胞部分和MIP-1 α活性在胃上皮细胞的培养物中未检测到。这一观察结果也得到了双重免疫荧光显微镜研究的证实，其中大多数（>90%）MIP-1 α阳性浸润细胞是CD 68（+）巨噬细胞。这项研究表明，MIP-1 α的合成和分泌增加，幽门螺杆菌感染的胃窦粘膜和粘膜巨噬细胞是主要的细胞类型负责这一现象。
Mucosal chemokines are considered to be important in the pathogenesis of Helicobacter pylori-associated gastritis. The aims of this study are to examine the levels of macrophage inflammatory protein-1 alpha (MIP-1 alpha) in organ cultures, the expression of MIP-1 alpha mRNA and the cellular source of MIP-1 alpha, using the antral mucosal specimens obtained from H. pylori-positive and -negative patients. Enzyme-linked immunosorbent assay was used to measure the levels of MIP-1 alpha in organ cultures of mucosal tissues and cell cultures of fractionated mucosal cells. The expression of MIP-1 alpha mRNA and protein was analysed in fresh biopsy tissues with reverse transcriptase-polymerase chain reaction (RT-PCR) and double immunofluorescence microscopy, respectively. The mucosal specimens obtained from H. pylori-positive patients exhibited significantly higher values of MIP-1 alpha activity in organ cultures with increased numbers of CD68(+) macrophages, myeloperoxidase(+) neutrophils and mononuclear cells in the lamina propria compared with those from H. pylori-negative patients. The RT-PCR analysis detected MIP-1 alpha mRNA in more than 50% of the specimens with H. pylori infection, but not in those without infection. In cell cultures, the macrophage fraction contained substantially higher amounts of MIP-1 alpha on a per cell basis than the lymphocyte fraction and MIP-1 alpha activity was not detected in cultures of gastric epithelial cells. This observation was also confirmed by a double immunofluorescence microscopic study in which most (>90%) MIP-1 alpha-positive infiltrating cells were CD68(+) macrophages. This study indicates that synthesis and secretion of MIP-1 alpha are increased in H, pylori-infected antral mucosa and that mucosal macrophages are the main cell type responsible for this phenomenon.