Low-Potential Photoelectrochemical Biosensing Using Porphyrin-Functionalized TiO2 Nanoparticles
Low-Potential Photoelectrochemical Biosensing Using Porphyrin-Functionalized TiO2 Nanoparticles
复制标题
使用卟啉功能化 TiO2 纳米粒子的低电位光电化学生物传感
DOI：
10.1021/ac102070f
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发表时间：
2010-10-15
影响因子：
7.4
通讯作者：
Ju, Huangxian
中科院分区：
文献类型：
--
作者：
Tu, Wenwen;Dong, Yitong;Ju, Huangxian
利用卟啉功能化的二氧化钛纳米粒子构建了一种新型的光电化学生物传感平台，用于在相对较低的外加电位下检测生物分子。通过水溶性[meso-tetrakis（4-sulfonatophenyl）porphyrin] Fe（III）monochloride（FeTPPS）的磺酸基与TiO 2配位，制备了功能性TiO 2纳米粒子，并通过透射电子显微镜、接触角测量、拉曼光谱、X射线光电子能谱和紫外-可见吸收光谱对其进行了表征。功能纳米粒子在水中和氧化铟锡（ITO）表面表现出良好的分散性。所得的FeTPPS-TiO 2-修饰的ITO电极显示出在+0.2 V的光电流响应于380 nm的光激发，这可以通过空穴注入的FeTPPS的生物分子的氧化过程进一步敏化。以谷胱甘肽为模型，建立了一种低电位下灵敏的光电化学生物传感方法。在最佳条件下，建议的光电化学方法可以检测范围为0.05至2.4 mmol L-1的谷胱甘肽与0.03 mmol L-1的信噪比为3。该光电化学生物传感器对抗癌药物具有良好的特异性，可成功应用于谷胱甘肽注射液中还原型谷胱甘肽的检测，在光电化学生物传感方面具有良好的应用前景。
A novel photoelectrochemical biosensing platform for the detection of biomolecules at relatively low applied potentials was constructed using porphyrin-functionalized TiO2 nanoparticles. The functional TiO2 nanoparticles were prepared by dentate binding of TiO2 with sulfonic groups of water-soluble [meso-tetrakis(4-sulfonatophenyl)porphyrin] iron(III) monochloride (FeTPPS) and characterized by transmission electron microscopy; contact angle measurement; and Raman, X-ray photoelectron, and ultraviolet-visible absorption spectroscopies. The functional nanoparticles showed good dispersion in water and on indium tin oxide (ITO) surface. The resulting FeTPPS-TiO2-modified ITO electrode showed a photocurrent response at +0.2 V to a light excitation at 380 nm, which could be further sensitized through an oxidation process of biomolecules by the hole-injected FeTPPS. Using glutathione as a model, a methodology for sensitive photoelectrochemical biosensing at low potential was thus developed. Under optimal conditions, the proposed photoelectrochemical method could detect glutathione ranging from 0.05 to 2.4 mmol L-1 with a detection limit of 0.03 mmol L-1 at a signal-to-noise ratio of 3. The photoelectrochemical biosensor had an excellent specificity against anticancer drugs and could be successfully applied to the detection of reduced glutathione in gluthion injection, showing a promising application in photoelectrochemical biosensing.