Transient expression of VP2 in Nicotiana benthamiana and its use as a plant-based vaccine against Infectious Bursal Disease Virus
Transient expression of VP2 in Nicotiana benthamiana and its use as a plant-based vaccine against Infectious Bursal Disease Virus
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DOI：
10.1016/j.vaccine.2013.03.064
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发表时间：
2013-05-28
期刊：
影响因子：
5.5
通讯作者：
Berinstein, Analia
中科院分区：
文献类型：
--
作者：
Gomez, Evangelina;Soledad Lucero, Maria;Berinstein, Analia
传染性法氏囊病病毒（IBDV）是一种免疫抑制和高度接触性疾病的病原体，影响幼鸟。这种疾病在世界范围内的家禽业造成重大的经济损失。VP 2蛋白已用于在多种异源平台中开发亚单位疫苗。在这种情况下，本研究的目的是调查VP 2的表达和免疫原性使用实验性的植物为基础的疫苗对IBDV。结果表明，N.本塞姆氏叶允许VP 2的产生，而其构象表位没有明显变化。以剂量/加强方案用粗浓缩提取物肌内免疫的鸡产生了具有病毒中和能力的特异性体液应答。鉴于这些结果，植物疫苗在兽医领域占有一席之地似乎是合理的。因此，植物可以是产生针对IBDV的免疫原的适当选择系统。(c)2013爱思唯尔有限公司保留所有权利。
Infectious Bursal Disease Virus (IBDV) is the etiological agent of an immunosuppressive and highly contagious disease that affects young birds. This disease causes important economic losses in the poultry industry worldwide. The VP2 protein has been used for the development of subunit vaccines in a variety of heterologous platforms. In this context, the aim of this study was to investigate VP2 expression and immunogenicity using an experimental plant-based vaccine against IBDV. We determined that the agroinfiltration of N. benthamiana leaves allowed the production of VP2 with no apparent change on its conformational epitopes. Chickens intramuscularly immunized in a dose/boost scheme with crude concentrated extracts developed a specific humoral response with viral neutralizing ability. Given these results, it seems plausible for a plant-based vaccine to have a niche in the veterinary field. Thus, plants can be an adequate system of choice to produce immunogens against IBDV. (c) 2013 Elsevier Ltd. All rights reserved.