Advanced biopolymer-coated drug-releasing titania nanotubes (TNTs) implants with simultaneously enhanced osteoblast adhesion and antibacterial properties
Advanced biopolymer-coated drug-releasing titania nanotubes (TNTs) implants with simultaneously enhanced osteoblast adhesion and antibacterial properties
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DOI：
10.1016/j.colsurfb.2015.04.021
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发表时间：
2015-06-01
影响因子：
5.8
通讯作者：
Losic, Dusan
中科院分区：
文献类型：
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作者：
Kumeria, Tushar;Mon, Htwe;Losic, Dusan
在这里，我们报告先进的生物聚合物涂层的药物释放植入物的基础上，钛（Ti）具有二氧化钛纳米管（TNT）在其表面上的发展。这些TNT阵列通过电化学阳极氧化在钛表面上制造，然后加载和释放模型抗生素药物庆大霉素。这些TNT Ti样品的成骨细胞粘附和抗菌性能显着改善，通过加载抗菌有效载荷内的纳米管和修改其表面与两个生物聚合物涂层（PLGA和壳聚糖）。通过成骨细胞和革兰氏阳性菌模型（表皮葡萄球菌）的粘附和增殖研究，证实了这些药物释放TNT Ti样品的成骨细胞粘附和抗菌性能得到改善。通过荧光和扫描电子显微镜监测这些细胞在TNT-Ti样品上的粘附。结果揭示了这些生物聚合物涂层的释放药物的TNT-Ti基质促进成骨细胞粘附和增殖的能力，同时通过阻碍其增殖和生物膜形成有效地防止细菌定植。所提出的方法可以克服与钛基植入物上的细菌感染相关的固有问题，同时能够开发具有增强和协同抗菌功能和骨细胞促进作用的骨科植入物。皇冠版权所有（C）2015由Elsevier B. V.出版。保留所有权利。
Here, we report on the development of advanced biopolymer-coated drug-releasing implants based on titanium (Ti) featuring titania nanotubes (TNTs) on its surface. These TNT arrays were fabricated on the Ti surface by electrochemical anodization, followed by the loading and release of a model antibiotic drug, gentamicin. The osteoblastic adhesion and antibacterial properties of these TNT Ti samples are significantly improved by loading antibacterial payloads inside the nanotubes and modifying their surface with two biopolymer coatings (PLGA and chitosan). The improved osteoblast adhesion and antibacterial properties of these drug-releasing TNT Ti samples are confirmed by the adhesion and proliferation studies of osteoblasts and model Gram-positive bacteria (Staphylococcus epidermidis). The adhesion of these cells on TNT-Ti samples is monitored by fluorescence and scanning electron microscopies. Results reveal the ability of these biopolymer-coated drug-releasing TNT-Ti substrates to promote osteoblast adhesion and proliferation, while effectively preventing bacterial colonization by impeding their proliferation and biofilm formation. The proposed approach could overcome inherent problems associated with bacterial infections on Ti-based implants, simultaneously enabling the development of orthopedic implants with enhanced and synergistic antibacterial functionalities and bone cell promotion. Crown Copyright (C) 2015 Published by Elsevier B.V. All rights reserved.