MUC17, a novel membrane-tethered mucin
MUC17, a novel membrane-tethered mucin
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DOI：
10.1006/bbrc.2002.6475
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发表时间：
2002-03-01
影响因子：
3.1
通讯作者：
Kim, YS
中科院分区：
文献类型：
--
作者：
Gum, JR;Crawley, SC;Kim, YS
膜粘蛋白在上皮细胞中具有多种功能，包括细胞保护、转移过程中的外渗、维持管腔结构和信号转导。在本文中，我们描述了在正常肠中表达的一个大的膜粘蛋白。这种新的粘蛋白被命名为MUC17，它包含一个延伸的、重复的细胞外糖基化结构域和一个羧基末端，它具有两个egf样结构域、一个SEA模块结构域、一个跨膜结构域和一个具有潜在丝氨酸和酪氨酸磷酸化位点的细胞质结构域。RNA印迹分析和原位杂交表明MUC17在选定的胰腺癌和结肠癌细胞系以及肠吸收细胞中表达。辐射杂交定位MUC17定位在染色体7q22上，与其他三个膜粘蛋白基因MUC3A、MUC3B和MUC12非常接近。因此，这些膜粘蛋白共同存在于一个基因簇中，但在不同的组织中表达，并且可能具有不同的功能。
Membrane mucins have several functions in epithelial cells including cytoprotection, extravasation during metastases, maintenance of luminal structure, and signal transduction. In this paper we describe a large membrane mucin expressed in the normal intestine. This novel mucin, designated MUC17, contains an extended, repetitive extracellular glycosylation domain and a carboxyl terminus with two EGF-like domains, a SEA module domain, a transmembrane domain, and a cytoplasmic domain with potential serine and tyrosine phosphorylation sites. RNA blot analysis and in situ hybridization indicates that MUC17 is expressed in select pancreatic and colon cancer cell lines and in intestinal absorptive cells. Radiation hybrid mapping localized MUC17 to chromosome 7q22 where it resides in close proximity with three other membrane mucin genes, MUC3A, MUC3B, and MUC12. Thus, these membrane mucins reside together in a gene cluster, but are expressed in different tissues and are likely to have different functions as well.