IL-1 beta regulates the expression of the Gi2 alpha gene via lipid mediators in guinea pig tracheal muscle.
IL-1 beta regulates the expression of the Gi2 alpha gene via lipid mediators in guinea pig tracheal muscle.
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IL-1 beta 通过豚鼠气管肌肉中的脂质介质调节 Gi2 α 基因的表达。
DOI：
10.1006/bbrc.1994.2408
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发表时间：
1994
影响因子：
3.1
通讯作者：
Hasegawa,S
中科院分区：
文献类型：
--
作者：
Hirata,F;Lee,JY;Sakamoto,T;Nomura,A;Uchida,Y;Hirata,A;Hasegawa,S
人重组IL-1β与豚鼠离体肌标本孵育后，GI2α表达水平呈时间和剂量依赖性增加，Gsα表达水平无明显变化。脂氧合酶(NDGA)、环氧合酶抑制剂(吲哚美辛)和PAF拮抗剂(SC47014A)可部分阻断这种增加，而U46619(血栓烷A2模拟物)、LTD4、15-HETE和PAF则部分地模拟这种增加。抗磷脂酶A2抗血清几乎完全抑制IL-1β诱导的GI2α的表达，而预免疫血清则无明显作用。从这些观察结果中，我们认为IL-1β首先诱导II型炎性磷脂酶A2的合成和释放，进而刺激GI2α基因的表达，从而产生各种脂质介质。
When isolated guinea pig muscle preparations were incubated with human recombinant IL-1β, mRNA level of Gi2αbut not of Gsαincreased in a time and dose dependent manner. The increase was partially blocked by inhibitors of lipoxygenase (NDGA) and cycleoxygenase (indomethacin) and PAF antagonist (SC47014A), while U46619 (thromboxane A2mimetic), LTD4, 15-HETE and PAF partially mimicked it. The IL-1β induced Gi2αexpression was almost completely inhibited by anti-phospholipase A2antiserum, whereas preimmune serum had no apparent effects. From these observations, we suggest that IL-1β first induces the synthesis and release of Type II inflammatory phospholipase A2, which in turn stimulates the expression of Gi2αgeneviaproduction of various lipid mediators.