Analysis of degradation products of nitrogen mustards via hydrophilic interaction liquid chromatography-tandem mass spectrometry
Analysis of degradation products of nitrogen mustards via hydrophilic interaction liquid chromatography-tandem mass spectrometry
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DOI：
10.1016/j.chroma.2019.05.015
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发表时间：
2019-09-27
影响因子：
4.1
通讯作者：
Uchiyama, Masanobu
中科院分区：
文献类型：
--
作者：
Otsuka, Mai;Miyaguchi, Hajime;Uchiyama, Masanobu
建立了氮芥降解产物乙醇胺的亲水作用液相色谱-串联质谱（HILIC-MS/MS）定性定量分析方法。用这种方法，HILIC柱上的高度亲水性EA的保留时间是足够的（保留时间：甲基二乙醇胺，12.2分钟;乙基二乙醇胺，11.2分钟;和三乙醇胺，9.5分钟）和EA的分析比报告的HILIC-MS/MS方法更有效。该方法对血清和尿液中甲基二乙醇胺和乙基二乙醇胺的检出限为15-20 ng/mL。通过涉及尿液和血清的回收率试验评价了该方法对真实的样品的适用性，并对该方法进行了验证。基于密度泛函理论计算讨论了EA的MS/MS裂解。(C)2019 Elsevier B. V.版权所有。
A hydrophilic interaction liquid chromatography-tandem mass spectrometry (HILIC-MS/MS) method was developed for qualitative and quantitative analysis of ethanolamines (EAs), which are nitrogen mustard degradation products. With this method, the retention times of the highly hydrophilic EAs on the HILIC column were sufficient (retention times: methyl diethanolamine, 12.2 min; ethyl diethanolamine, 11.2 min; and triethanolamine, 9.5 min) and the EAs were analyzed more efficiently than with reported HILIC-MS/MS methods. The detection limits of methyl diethanolamine and ethyl diethanolamine in serum and urine using this approach were 15-20 ng/mL. The suitability of the method for real samples was evaluated via recovery tests involving urine and serum, and the method was validated. The MS/MS fragmentation of EAs was discussed based on density functional theory calculations. (C) 2019 Elsevier B.V. All rights reserved.