The DCP2 protein is required for mRNA decapping in Saccharomyces cerevisiae and contains a functional MutT motif
The DCP2 protein is required for mRNA decapping in Saccharomyces cerevisiae and contains a functional MutT motif
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DOI：
10.1093/emboj/18.19.5411
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发表时间：
1999-10-01
期刊：
影响因子：
11.4
通讯作者：
Parker, R
中科院分区：
文献类型：
--
作者：
Dunckley, T;Parker, R
酵母中mRNA降解的主要途径是通过转录体的去腺苷酸化、去帽和随后的5'至3'核酸外切降解发生。为了鉴定控制由DCP 1基因编码的去帽酶活性的蛋白质，我们分离了温度敏感性dcp 1 -2等位基因的高拷贝抑制基因，称为DCP 2，过表达Dcp 2 p部分抑制dcy 1 -2脱帽缺陷。此外，Dcp 2蛋白对于正常mRNA和通过mRNA监视途径降解的异常转录物的脱帽都是必需的。Dcp 2蛋白含有MutT基序，其在一类焦磷酸酶中发现。突变分析表明，Dcp 2 p中含有MutT基序的区域是Dcp 2 p在mRNA脱帽中发挥作用的必要和充分条件，Dcp 2 p还与DCP 1脱帽酶共免疫沉淀，并且是产生具有酶活性的脱帽酶所必需的。这些结果表明，Dcp 1 p与Dcp 2 p的直接或间接相互作用是产生具有活性的脱帽酶所必需的。可能在需要焦磷酸键水解的过程中。
The major pathway of mRNA degradation in yeast occurs through deadenylation, decapping and subsequent 5' to 3' exonucleolytic decay of the transcript body, To identify proteins that control the activity of the decapping enzyme, which is encoded by the DCP1 gene, we isolated a high-copy suppressor of the temperature-sensitive dcp1-2 allele, termed DCP2, Overexpression of Dcp2p partially suppressed the dcy1-2 decapping defect. Moreover, the Dcp2 protein was required for the decapping of both normal mRNAs and aberrant transcripts that are degraded by the mRNA surveillance pathway, The Dcp2 protein contains a MutT motif, which is found in a class of pyrophosphatases. Mutational analyses indicated that the region of Dcp2p containing the MutT motif is necessary and sufficient for Dcp2p's function in mRNA decapping, The Dcp2p also coimmunoprecipitates with the DCP1 decapping enzyme and is required for the production of enzymatically active decapping enzyme, These results suggest that direct or indirect interaction of Dcp1p with Dcp2p is required for the production of active decapping enzyme, perhaps in a process requiring the hydrolysis of a pyrophosphate bond.