A novel magnetic resonance-based method to measure gene expression in living cells
A novel magnetic resonance-based method to measure gene expression in living cells
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DOI：
10.1093/nar/gkl135
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发表时间：
2006-01-01
影响因子：
14.9
通讯作者：
Kokubo, T
中科院分区：
文献类型：
--
作者：
Ki, S;Sugihara, F;Kokubo, T
在单细胞和多细胞真核生物中，复杂的基因调控机制促进了从细胞分裂到形态分化的广泛的生物过程。为了充分了解这些生物过程中涉及的基因调控网络，需要在活生物体中真实的时间内确定数千个基因表达的空间和时间模式。目前可用的技术不足以实现这一目标，然而，基于磁共振（MR）成像的新方法可能是特别有用的不透明组织中的基因表达的灵敏检测。本报告描述了一种新的报告基因系统，动态和定量地监测基因表达，在酵母细胞中，通过测量积累的无机聚磷酸盐（聚P）使用MR光谱（MRS）或MR光谱成像（MRI）。由于该系统是完全非侵入性的，并且不需要外源底物，因此它也是研究多细胞生物中基因表达的有力工具。
In unicellular and multicellular eukaryotes, elaborate gene regulatory mechanisms facilitate a broad range of biological processes from cell division to morphological differentiation. In order to fully understand the gene regulatory networks involved in these biological processes, the spatial and temporal patterns of expression of many thousands of genes will need to be determined in real time in living organisms. Currently available techniques are not sufficient to achieve this goal; however, novel methods based on magnetic resonance (MR) imaging may be particularly useful for sensitive detection of gene expression in opaque tissues. This report describes a novel reporter gene system that monitors gene expression dynamically and quantitatively, in yeast cells, by measuring the accumulation of inorganic polyphosphate (polyP) using MR spectroscopy (MRS) or MR spectroscopic imaging (MRI). Because this system is completely non-invasive and does not require exogenous substrates, it is a powerful tool for studying gene expression in multicellular organisms, as well.