Expression of olfactory receptors in the cribriform mesenchyme during prenatal development
Expression of olfactory receptors in the cribriform mesenchyme during prenatal development
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DOI：
10.1016/j.modgep.2004.02.004
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发表时间：
2004-09-01
影响因子：
1.2
通讯作者：
Conzelmann, S
中科院分区：
文献类型：
--
作者：
Schwarzenbacher, K;Fleischer, J;Conzelmann, S
嗅觉受体（ORs）在鼻上皮的感觉神经元中表达，在那里它们被认为参与识别合适的气味化合物和引导向外生长的轴突朝向嗅球中的适当肾小球。在发育过程中，在非感觉组织中也发现了一些嗅觉受体亚型，包括未来的嗅觉上皮和发育中的端脑之间的筛状间充质，但这是否是OR的典型现象尚不清楚。监测几种受体亚型表达的起始和时间过程显示，OR的“上皮外”表达发生得非常早和短暂，特别是在胚胎阶段E10.25和E14.0之间。在后期，观察到受体表达细胞的进行性损失。分子表型分析表明，在筛状间充质中的受体表达细胞共表达关键元件，包括Ga（olf）、ACIII和OMP，其是鼻上皮中嗅觉神经元的特征。对转基因OMP/GFP小鼠的研究表明，“上皮外”OMP/GFP阳性细胞位于从鼻上皮到假定嗅球的轴突束附近。此外，这些细胞主要位于轴突成束并改变方向朝向前脑前部的地方。(C)2004 Elsevier B. V.保留所有权利。
Olfactory receptors (ORs) are expressed in sensory neurons of the nasal epithelium, where they are supposed to be involved in the recognition of suitable odorous compounds and in the guidance of outgrowing axons towards the appropriate glomeruli in the olfactory bulb. During development, some olfactory receptor subtypes have also been found in non-sensory tissues, including the cribriform mesenchyme between the prospective olfactory epithelium and the developing telencephalon, but it is elusive if this is a typical phenomenon for ORs. Monitoring the onset and time course of expression for several receptor subtypes revealed that 'extraepithelial' expression of ORs occurs very early and transiently, in particular between embryonic stages E10.25 and E14.0. In later stages, a progressive loss of receptor expressing cells was observed. Molecular phenotyping demonstrated that the receptor expressing cells in the cribriform mesenchyme co-express key elements, including Galpha(olf), ACIII and OMP, characteristic for olfactory neurons in the nasal epithelium. Studies on transgenic OMP/GFP-mice showed that 'extraepithelial' OMP/GFP-positive cells are located in close vicinity to axon bundles projecting from the nasal epithelium to the presumptive olfactory bulb. Moreover, these cells are primarily located where axons fasciculate and change direction towards the anterior part of the forebrain. (C) 2004 Elsevier B.V. All rights reserved.