Nonvariant antigens limited to bloodstream forms of Trypanosoma brucei brucei and Trypanosoma brucei rhodesiense.
Nonvariant antigens limited to bloodstream forms of Trypanosoma brucei brucei and Trypanosoma brucei rhodesiense.
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非变异抗原仅限于布氏锥虫和罗得西亚布氏锥虫的血流形式。
DOI：
10.1111/j.1550-7408.1984.tb05499.x
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Honigberg,BM
中科院分区：
文献类型：
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作者：
Beat,DA;Stanley,HA;Choromański,L;MacDonald,AB;Honigberg,BM
通过免疫扩散和免疫电泳首次证明了仅限于布氏锥虫和罗得西亚布氏锥虫血流形式的非变异抗原（NVA）的存在。非克隆和克隆群体用于制备兔多克隆抗血清和用于免疫反应的抗原。 NVA 在超免疫兔血清（吸附有原环形式以消除针对血流形式和原环阶段常见抗原的抗体）与以异源变体特异性抗原（VSA）为特征的锥虫反应的系统中可以得到最好的显示。本研究中证明的 NVA 很可能与 VSA 的常见部分不同。正如活体锥虫实验所表明的那样，至少一部分 NVA 似乎位于血流形式的表面。在这些涉及定量间接荧光抗体测试的实验中，异源系统（即与抗 AmTat 1.1 血清一起孵育的 ETat 5 锥虫）记录的荧光量相当于用同源抗血清处理的 AmTat 1.1 血流形式发出的荧光的~3.0%。显然，T 表面仅存在少量 NVA。除了NVA之外，电泳结果还表明属于不同T的前循环阶段之间存在抗原差异。布鲁斯斯托克斯。
The presence of nonvariant antigens (NVAs) limited to bloodstream forms ofTrypanosoma brucei bruceiandTrypanosoma brucei rhodesiensewas demonstrated for the first time by immunodiffusion and Immunoelectrophoresis. Noncloned and cloned populations were employed in preparation of polyclonal antisera in rabbits and of antigens to be used in the immunologic reactions. The NVAs could be shown best in systems in which hyperimmune rabbit sera (adsorbed with procyclic forms to eliminate antibodies against antigens common to bloodstream form and procyclic stages) were reacted with trypanosomes characterized by heterologous variant‐specific antigens (VSAs).The NVAs demonstrated in this study are very likely different from the common parts of VSAs. As has been suggested by experiments with living trypanosomes, at least a part of the NVAs appears to be located on the surface of the bloodstream forms. In these experiments involving the quantitative indirect fluorescent antibody test, the amount of fluorescence recorded for the heterologous system, i.e. ETat 5 trypanosomes incubated with anti‐AmTat 1.1 serum, equalled ∼3.0% of the fluorescence emitted by the AmTat 1.1 bloodstream forms treated with their homologous antiserum. Evidently, only small amounts of NVAs are present on the surfaces ofT. bruceibloodstream forms.In addition to the NVAs, the electrophoresis results suggested the presence of antigenic differences between procyclic stages belonging to differentT. bruceistocks.