Essential requirement of an invariant V alpha 14 T cell antigen receptor expression in the development of natural killer T cells.
Essential requirement of an invariant V alpha 14 T cell antigen receptor expression in the development of natural killer T cells.
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DOI：
10.1073/pnas.93.20.11025
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发表时间：
1996-10
影响因子：
11.1
通讯作者：
M. Taniguchi;H. Koseki;T. Tokuhisa;K. Masuda;H. Sato;E. Kondo;T. Kawano;J. Cui;A. Perkes;S. Koyasu;Y. Makino
中科院分区：
文献类型：
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作者：
M. Taniguchi;H. Koseki;T. Tokuhisa;K. Masuda;H. Sato;E. Kondo;T. Kawano;J. Cui;A. Perkes;S. Koyasu;Y. Makino
NK1.1+T[自然杀伤(NK)T]细胞表达由Vα14和Jα281片段编码的不变T细胞抗原受体α链(TCRα)，并与有限数量的Vβ相关，主要是Vβ8.2。在缺乏内源性TCRα表达的转基因小鼠中，表达不变的Vα14/Jα281，而不是Vα1，TCR阻止了常规Tαβ细胞的发育，并导致Vα14 NK T细胞的优先发育，提示不变Vα14 TCR在NK T细胞发育中起先决条件作用。在Vβ8.2而非Bβ3转基因小鼠中，可检测到两种不同CD3 epsilon表达的NK T细胞：CD3 epsilon(DIM)和CD3 epsilon(High)。CD3 epsilon(高)NK T细胞表达表面Vα14/V beta 8 TCR，提示成熟细胞类型，而CD3 epsilon(DIM)NK T细胞表达V beta 8而Vα14 TCR不表达，CD3 epsilon(DIM)无明显CD3 epsilon表达。而后者表达重组激活基因(RAG-1和RAG-2)，仅在前体或未成熟T细胞系中表达，并在胞浆中表达CD3 epsilon mRNA，提示CD3 epsilon(DIM)NK T细胞是Vα14 NK T细胞的前体。
NK1.1+ T [natural killer (NK) T] cells express an invariant T cell antigen receptor alpha chain (TCR alpha) encoded by V alpha 14 and J alpha 281 segments in association with a limited number of V betas, predominantly V beta 8.2. Expression of the invariant V alpha 14/J alpha 281, but not V alpha 1, TCR in transgenic mice lacking endogenous TCR alpha expression blocks the development of conventional T alpha beta cells and leads to the preferential development of V alpha 14 NK T cells, suggesting a prerequisite role of invariant V alpha 14 TCR in NK T cell development. In V beta 8.2 but not B beta 3 transgenic mice, two NK T cells with different CD3 epsilon expressions, CD3 epsilon(dim) and CD3 epsilon(high), can be identified. CD3 epsilon(high) NK T cells express surface V alpha 14/V beta 8 TCR, indicating a mature cell type, whereas CD3 epsilon(dim) NK T cells express V beta 8 without V alpha 14 TCR and no significant CD3 epsilon expression (CD3 epsilon(dim)) on the cell surface. However, the latter are positive for recombination activating gene (RAG-1 and RAG-2) mRNA, which are only expressed in the precursor or immature T cell lineage, and also possess CD3 epsilon mRNA in their cytoplasm, suggesting that CD3 epsilon(dim) NK T cells are the precursor of V alpha 14 NK T cells.