Establishment and characterization of an epithelial cell line with quasi-normal chromosomes from a tubular adenoma of a familial polyposis coli patient.
Establishment and characterization of an epithelial cell line with quasi-normal chromosomes from a tubular adenoma of a familial polyposis coli patient.
复制标题
DOI：
10.1111/j.1349-7006.1991.tb01820.x
复制
发表时间：
1991-03
期刊：
影响因子：
--
通讯作者：
Kitagawa T
中科院分区：
文献类型：
--
作者：
Kawaguchi T;Miyaki M;Masui T;Watanabe M;Ohta H;Maruyama M;Utakoji T;Kitagawa T
一种名为 FPCK-1 的上皮细胞系是从男性家族性结肠息肉病 (FPC) 患者的管状腺瘤中建立的。 FPCK-1细胞生长非常缓慢，产生大量粘液，并且在培养物中稳定维持了3年。在软琼脂或裸鼠中均没有明显的生长，FPCK-1 细胞呈现正常的雄性核型，并且没有显示 5、17、18 和 22 号染色体上特定位点的丢失，据报道，这些位点在人类结肠癌中经常丢失。这些细胞既没有 K-ras 基因密码子 12 上的点突变，也没有 myc、c-H-ras 和/或 c-K-ras 基因的基因扩增。因此，这些结果表明 FPC 患者中存在迄今为止未知的导致腺瘤发展的致病事件。 FPCK-1 细胞系对于进一步分析研究人类结肠癌发生的多个步骤应该是有用的。
An epithelial cell line designated FPCK‐1 has been established from a tubular adenoma developing in a male familial polyposis coli (FPC) patient. The FPCK‐1 cells grow very slowly with adundant mucus production and have been maintained stably for 3 years in culture. No growth was evident either in soft agar or nude mice, FPCK‐1 cells present a normal male karyotype and do not show loss of specific loci on chromosomes 5,17,18, and 22 which have been reported to be lost frequently in human colon carcinomas. The cells have neither a point mutation on codon 12 of K‐ras gene nor gene amplification of myc, c‐H‐ras, and/or c‐K‐ras genes. These results thus suggest the existence of hitherto unknown causative event(s) underlying adenoma development in FPC patients. The FPCK‐1 cell line should prove useful for further analytical investigation of the multiple steps involved in human colon carcinogenesis.