Amplified Magnetic Resonance Sensing via Enzyme-Mediated Click Chemistry and Magnetic Separation
Amplified Magnetic Resonance Sensing via Enzyme-Mediated Click Chemistry and Magnetic Separation
复制标题
通过酶介导的点击化学和磁分离放大磁共振传感
DOI：
10.1021/acs.analchem.9b03550
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发表时间：
2019
影响因子：
7.4
通讯作者：
Chen Yiping
中科院分区：
文献类型：
--
作者：
Wu Long;Xianyu Yunlei;Wang Zhilong;Dong Yongzhen;Hu Xiaobo;Chen Yiping
磁共振传感（Magnetic Resonance Sensing，MRS）是一种前处理简单的生物传感器，传统的MRS灵敏度较低，不能用于痕量目标的分析。在这项工作中，我们采用碱性磷酸酶介导的点击化学作为信号转换和放大技术，有效地增强了放大MRS的磁信号。我们概述了两种策略，通过调节聚集状态或基于点击化学触发的纳米颗粒聚集的磁性探针的数量和它们的磁性分离能力。实验结果表明，基于磁性纳米探针数的调制和磁分离的组合胜过基于纳米探针聚集的策略，以提高灵敏度，为基于MRS的小分子分析提供了一个有吸引力的平台。为了证明应用，我们应用这种基于点击化学的方法来检测克伦特罗，一种非法使用的药物，以促进牲畜的生长。该方法的线性检测范围为0.1 ng mL-1 ~ 500 ng mL-1，检测限为0.02 ng mL-1，表明点击化学介导的扩增MRS可以作为一种有前途的磁性平台，用于检测真实样品中痕量的小分子。
Magnetic resonance sensing (MRS) assays with simple pretreatment have drawn increasing attention for the development of biosensors, whereas conventional MRS is not competent for analyzing trace targets due to its relative low sensitivity. In this work, we employ alkaline phosphatase-mediated click chemistry as a signal transformation and amplification technique that effectively enhances the magnetic signal for amplified MRS. We outline two strategies by modulating either the aggregation state or the number of the magnetic probes based on click chemistry-triggered aggregation of nanoparticles and their ability for magnetic separation. The experimental results show that the combination of magnetic nanoprobe number-based modulation and magnetic separation outcompetes nanoprobe aggregation-based strategy for an enhanced sensitivity, providing an attractive platform for MRS-based analysis of small molecules. For proof of application, we apply this click chemistry-based approach for the detection of clenbuterol, an illegally used drug to promote growth in livestock. It shows a linear detection range from 0.1 ng mL–1to 500 ng mL–1with a limit of detection of 0.02 ng mL–1, revealing that the click chemistry-mediated amplified MRS can act as a promising magnetic platform to detect trace levels of small molecules in authentic samples.