Lipopolysaccharides induce p8 mRNA expression in vivo and in vitro
Lipopolysaccharides induce p8 mRNA expression in vivo and in vitro
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DOI：
10.1006/bbrc.1999.0953
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发表时间：
1999-07-14
影响因子：
3.1
通讯作者：
Iovanna, JL
中科院分区：
文献类型：
--
作者：
Jiang, YF;Vaccaro, MI;Iovanna, JL
系统性脂多糖内毒素与急性胰腺损伤有关。损伤是否由LPS与胰腺细胞的直接相互作用引起尚不清楚。我们通过监测p8在体内和体外对LPS的反应来解决这个问题，因为p8蛋白的过表达是胰腺侵袭的敏感标志。在体内研究中，大鼠单次腹腔注射LPS后，在不同时间处死大鼠，处理胰腺、肝脏、肾脏、肺、脑和肠道进行RNA制备。在体外，用0.1、1或10 μ g/ml LPS培养胰腺腺泡AR4-2J细胞6、12或24小时，用Northern blotting检测p8 mRNA的表达。在体内，在治疗12小时后，胰腺中的浓度明显升高，24小时后仍保持升高。肾脏和肝脏中也有诱导，分别在6和12小时达到最大值，但在肺、脑和肠中没有。在AR4-2J细胞中，基底p8 mRNA表达极低，LPS处理后呈时间和剂量依赖性增加。LPS诱导的p8 mRNA在体内过表达证实了内毒素血症对胰腺的不良影响，其在体外的过表达证实了LPS与胰腺细胞的直接相互作用。(C) 1999学术出版社。
Systemic LPS endotoxin is associated with acute pancreatic damage. Whether damage results from direct interaction of LPS with pancreatic cells is unknown. We addressed that question by monitoring p8 expression in reponse to LPS, in vivo and in vitro, because overexpression of the p8 protein is a sensitive marker of pancreatic agression. For in vivo studies, rats were sacrificed at different times after a single intraperitoneal injection of LPS, and pancreas, liver, kidney, lung, brain, and intestine were processed for RNA preparation. In vitro, pancreatic acinar AR4-2J cells were cultivated with 0.1, 1, or 10 mu g/ml LPS for 6, 12, or 24 h. p8 mRNA expression was monitored by Northern blotting. In vivo, it was strongly increased in the pancreas after 12 h of treatment and remained elevated after 24 h. It was also induced in kidney and liver, with a maximum at 6 and 12 h, respectively, but not in lung, brain, or intestine. In AR4-2J cells, basal p8 mRNA expression was very low and increased in a time- and dose-dependent manner after treatment with LPS. LPS-induced overexpression of p8 mRNA in vivo confirmed the adverse effect of endotoxemia on pancreas and its overexpression in vitro demonstrated a direct interaction of LPS with pancreatic cells. (C) 1999 Academic Press.