RNA interference-mediated knockdown of the transcription factor Kruppel homologue 1 suppresses vitellogenesis in Chilo suppressalis
RNA interference-mediated knockdown of the transcription factor Kruppel homologue 1 suppresses vitellogenesis in Chilo suppressalis
复制标题
RNA干扰介导的转录因子Kruppel同源物1的敲低抑制二化螟卵黄发生
DOI：
10.1111/imb.12617
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发表时间：
2019-10-21
影响因子：
2.6
通讯作者：
Li, Y.
中科院分区：
文献类型：
--
作者：
Tang, Y.;He, H.;Li, Y.
全变态昆虫的卵黄发生涉及脂肪体在发育中的卵母细胞中产生和分泌卵黄蛋白原（Vg）和其他卵黄蛋白前体，所有这些主要由保幼激素（JH）协调。 Kruppel 同源物 1 (Kr-h1) 是一种锌指转录因子，已被证明是 JH 早期诱导基因并有助于繁殖。然而，人们对 Kr-h1 在昆虫繁殖中的确切分子功能知之甚少。在当前的研究中，我们使用臭名昭著的害虫二化螟作为模型系统来研究 Kr-h1 在雌性生殖中的作用。对二化螟 Kr-h1 的克隆和测序表明，它与其他鳞翅目昆虫的同源物具有高度的同一性。此外，RNA干扰介导的CsKr-h1敲低显着降低了脂肪体内Vg的转录，显着减少了卵黄蛋白沉积，还损害了卵母细胞成熟和卵巢发育，表明Kr-h1对于二化螟正常卵黄发生是不可或缺的。基于这些结果，我们得出结论，Kr-h1 对于昆虫的繁殖至关重要，针对该基因可能成为抑制水稻害虫的新方法。
Vitellogenesis in holometabolous insects involves the production and secretion of vitellogenin (Vg) and other yolk protein precursors in developing oocyte by the fat body, all of which is predominantly orchestrated by juvenile hormone (JH). Kruppel homologue 1 (Kr-h1) is a zinc finger transcription factor that has been demonstrated to be a JH-early inducible gene and to contribute to reproduction. However, the exact molecular function of Kr-h1 in insect reproduction is poorly understood. In the current study, we used the notorious pest Chilo suppressalis as a model system to investigate the role of Kr-h1 in female reproduction. Cloning and sequencing C. suppressalis Kr-h1 revealed that it shares high identity with its homologues from other lepidopteran insects. Moreover, RNA interference-mediated knockdown of CsKr-h1 substantially reduced the transcription of Vg in the fat body, dramatically decreased yolk protein deposition and also impaired oocyte maturation and ovarian development, indicating that Kr-h1 is indispensable for normal vitellogenesis in C. suppressalis. Based on these results, we conclude that Kr-h1 is crucial to reproduction in insects and that targeting this gene could potentially be a new way to suppress rice pests.