Primary structure of the Leishmania donovani small subunit ribosomal RNA coding region.
Primary structure of the Leishmania donovani small subunit ribosomal RNA coding region.
复制标题
杜氏利什曼原虫小亚基核糖体 RNA 编码区的一级结构。
DOI：
10.1093/nar/16.14.7198
复制
发表时间：
1988
影响因子：
14.9
通讯作者：
Sogin,ML
中科院分区：
文献类型：
--
作者：
Looker,D;Miller,LA;Elwood,HJ;Stickel,S;Sogin,ML
杜氏利什曼原虫苏丹I株核糖体小亚基RNA编码区包含3个大小分别为3.7kb、1.0kb和56bp的EcoRl/EcoRl限制性内切酶片段。将3.7kb和1kb片段克隆到pTZ 18U载体中，并将含有56kb片段的450bHindIII/Pst1片段与3.7kb和1.0kb的EcoRl片段重叠，直接克隆到M13噬菌体mpl8中。通过使用与真核小亚基rRNA基因(2)中高度保守的区域互补的寡核苷酸引物启动双脱氧链末端测序方案(1)来确定编码区两条链的完整序列。The L_donovani sequence is 2204 nucleotides long and is more closely related to Crithidia fasciculata(97%)(3)than to Trypanosoma brucei(84%)(4).GAUCUGGUUG AUUCUGCCAG UAGUCAUAUG CUUGUUUCAA GGACUUAGCC AUGCAUGCCU GAAUCUGCGC AUGGCUCAUU ACAUCAGACG UAAUCUGCCG CAAAAAUCUU GCGGUUUCCG AAACGCCAAG CUAAUACAUG AACCAACCGG GUGUUCUCCA CUCCAGACGG UGGGCAACCA AAUGAAUGAC AGUAAAACCA AUGCCUUCAC UGGCAGUAAC ACCCAGCAGU GUUGACUCAA GCUUGUUCCG GCGUCUUUUG ACGAACAACU GCCCUAUCAG CUGGUGAUGG CCGUGUAGUG GAGCGGGGGA UUAGGGUUCG AUUCCGGAGA GGGAGCCUGA GAAAUAGCUA CCACUUCUAC AUUGCCCAAU GUCAAAACAA AACGAUGAGG CAGCGAAAAG AAAUAGAGUU GUCAGUCCAU GGGAUAUUUA AACCCAUCCA AUAUCGAGUA ACAAUUGGAG GACAAGUCUG GUGCCAGCAC AAAGCGUAUA UUAAUGCUGU UGCUGUUAAA GGGUUCGUAG UUGAACUGUG GGCUGUGCAG UCCAUGUCGG AUUUGGUGAC CCAGGCCCUU GCAGCCCGUG AACAUUCAAA GAAACAAGAA CUGAUUUACG CAUGUCAUGC AUGCCAGGGG GCGUCCGUGA UUUUUUACUG UGACUAAAGA UUGACUUGAA UUAGAAAGCA UGGGAUAACA AAGGAGCAGC CUCUAGGCUA CCGUUUCGGC UAUUGGAGAU UAUGGAGCUG UGCGACAAGU GCUUUCCCAU CGCAACCUCG GUUCGGUGUG GUGCGUCCGG UACGAGCUCC GGUUCGUCCG GCCGUAACGC CUUUUCAACU CACGGCCUCUCGGGGGAGAA CGUACUGGGG CGUCAGAGGU GAAAUUCUUA GACCGCACCA AGACGAACUA GAUACCUUCC UCAAUCAAGA ACCAAAGUGU GGAGAUCGAA GAUGAUUAGA GACCAUUGUA CACCCAUGAA UUGGGGAUCU UAUGGGCCGG CCUGCGGCAG GGUUUACCCU GUGUCCAGCA UUACGUAUCU UUUCUAUUCG GCCUUUACCG GCCACCCACG GGAAUAUCCU CAGCACGUUU CUUUGAGGUU ACAGUCUCAG GGGGGAGUAC UGGAGCGUGC GGUUUAAUUU GACUCAACAC CUUUCUCGAU UCCCUGAAUG GUGGUGCAUG GAGAUCCAAG CUGCCCAGUA GAAUUCAGAA GCAUUCGGUC GAAUUCUUCU CUGCGGGAUU CUCCUCAAUG UUCUGGGCGA CACGCGCACU AAAAGAGUGG GGAAACCCCG GAAUCACAUA UCUCGUAGGC GCAGCUCAUC AAACUGUGCC GGUGCAAUAC AGGUGAUCGG ACAGGCGGUG GUCGUAACAA GGUAGCUGUA GGUGAACCUG
The small subunit ribosomal RNA coding region for the Sudan I strain of Leishmania donovani is contained within three EcoRl/EcoRl restriction fragments 3.7 kb, 1.0 kb, and 56 bp in size. The 3.7 kb and 1 kb fragments were cloned into PTZ 18U plasmids and a 450 bp HindIII/Pstl which contains the 56 bp fragment and overlaps the 3.7 kb and 1.0 kb EcoRl fragments was directly cloned into M13 phage mpl8. Complete sequences for both strands of the coding region were determined by initiating the dideoxynucleotide chain termination sequencing protocols (1) with oligonucleotide primers that are complementary to highly conserved regions in eukaryotic small subunit rRNA genes (2). The L_ donovani sequence is 2204 nucleotides long and is more closely related to Crithidia fasciculata (97%)(3) than to Trypanosoma brucei (84%)(4).GAUCUGGUUG AUUCUGCCAG UAGUCAUAUG CUUGUUUCAA GGACUUAGCC AUGCAUGCCU GAAUCUGCGC AUGGCUCAUU ACAUCAGACG UAAUCUGCCG CAAAAAUCUU GCGGUUUCCG AAACGCCAAG CUAAUACAUG AACCAACCGG GUGUUCUCCA CUCCAGACGG UGGGCAACCA AAUGAAUGAC AGUAAAACCA AUGCCUUCAC UGGCAGUAAC ACCCAGCAGU GUUGACUCAA GCUUGUUCCG GCGUCUUUUG ACGAACAACU GCCCUAUCAG CUGGUGAUGG CCGUGUAGUG GAGCGGGGGA UUAGGGUUCG AUUCCGGAGA GGGAGCCUGA GAAAUAGCUA CCACUUCUAC AUUGCCCAAU GUCAAAACAA AACGAUGAGG CAGCGAAAAG AAAUAGAGUU GUCAGUCCAU GGGAUAUUUA AACCCAUCCA AUAUCGAGUA ACAAUUGGAG GACAAGUCUG GUGCCAGCAC AAAGCGUAUA UUAAUGCUGU UGCUGUUAAA GGGUUCGUAG UUGAACUGUG GGCUGUGCAG UCCAUGUCGG AUUUGGUGAC CCAGGCCCUU GCAGCCCGUG AACAUUCAAA GAAACAAGAA CUGAUUUACG CAUGUCAUGC AUGCCAGGGG GCGUCCGUGA UUUUUUACUG UGACUAAAGA UUGACUUGAA UUAGAAAGCA UGGGAUAACA AAGGAGCAGC CUCUAGGCUA CCGUUUCGGC UAUUGGAGAU UAUGGAGCUG UGCGACAAGU GCUUUCCCAU CGCAACCUCG GUUCGGUGUG GUGCGUCCGG UACGAGCUCC GGUUCGUCCG GCCGUAACGC CUUUUCAACU CACGGCCUCU CGGGGGAGAA CGUACUGGGG CGUCAGAGGU GAAAUUCUUA GACCGCACCA AGACGAACUA GAUACCUUCC UCAAUCAAGA ACCAAAGUGU GGAGAUCGAA GAUGAUUAGA GACCAUUGUA CACCCAUGAA UUGGGGAUCU UAUGGGCCGG CCUGCGGCAG GGUUUACCCU GUGUCCAGCA UUACGUAUCU UUUCUAUUCG GCCUUUACCG GCCACCCACG GGAAUAUCCU CAGCACGUUU CUUUGAGGUU ACAGUCUCAG GGGGGAGUAC UGGAGCGUGC GGUUUAAUUU GACUCAACAC CUUUCUCGAU UCCCUGAAUG GUGGUGCAUG GAGAUCCAAG CUGCCCAGUA GAAUUCAGAA GCAUUCGGUC GAAUUCUUCU CUGCGGGAUU CUCCUCAAUG UUCUGGGCGA CACGCGCACU AAAAGAGUGG GGAAACCCCG GAAUCACAUA UCUCGUAGGC GCAGCUCAUC AAACUGUGCC GGUGCAAUAC AGGUGAUCGG ACAGGCGGUG GUCGUAACAA GGUAGCUGUA GGUGAACCUG