Using oxidized carbon nanotubes as matrix for analysis of small molecules by MALDI-TOF MS
Using oxidized carbon nanotubes as matrix for analysis of small molecules by MALDI-TOF MS
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DOI：
10.1016/j.jasms.2005.03.009
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发表时间：
2005-06-01
影响因子：
3.2
通讯作者：
Guo, BC
中科院分区：
文献类型：
--
作者：
Pan, CS;Xu, SY;Guo, BC
以氧化碳纳米管为基质，采用基质辅助激光解吸电离飞行时间质谱仪(MALDI-TOF-MS)分析小分子物质。与非氧化型碳纳米管相比，氧化型碳纳米管在水中具有较高的溶解度，有利于样品的制备。氧化后的碳纳米管的基质层比未氧化的碳纳米管更均匀致密。在氧化碳纳米管表面，分析物的解吸/电离效率和样品点内和样点之间的峰强度的重现性大大提高。通过对氨基酸混合物的MALDI-TOF-MS分析，证明了氧化碳纳米管相对于α-氰基-4-羟基肉桂酸(CCA)和碳纳米管的优势。该基质已成功地用于合成羟丙基对环糊精的分析，在反应监测和产品质量控制方面具有很大的潜力。用该基质可实现药根碱和巴马汀的可靠定量，线性范围宽(1~100 ng/mL)，相对峰面积重现性好(RSD<10%)。用基质和标准加入法同时测定了黄连提取物中药根碱(8.65 mg/m L)和巴马汀(10.4 mg/m L)的含量。(C)2005年美国质谱学学会。
Oxidized carbon nanotubes are tested as a matrix for analysis of small molecules by matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS). Compared with nonoxidized carbon nanotubes, oxidized carbon nanotubes facilitate sample preparation because of their higher solubility in water. The matrix layer of oxidized carbon nanotubes is much more homogeneous and compact than that of nonoxidized carbon nanotubes. The efficiency of desorption/ionization for analytes and the reproducibility of peak intensities within and between sample spots are greatly enhanced on the surface of oxidized carbon nanotubes. The advantage of the oxidized carbon nanotubes in comparison with alpha-cyano-4-hydroxycinnamic acid (CCA) and carbon nanotubes is demonstrated by MALDI-TOF-MS analysis of an amino acid mixture. The matrix is successfully used for analysis of synthetic hydroxypropyl P-cyclodextrin, suggesting a great potential for monitoring reactions and for product quality control. Reliable quantitative analysis of jatrorrhizine and palmatine with a wide linear range (1-100 ng/mL) and good reproducibility of relative peak areas (RSD less than 10 %) is achieved using this matrix. Concentrations of jatrorrhizine (8.65 mg/mL) and palmatine (10.4 mg/mL) in an extract of Coptis chinensis Franch are determined simultaneously using the matrix and a standard addition method. (c) 2005 American Society for Mass Spectrometry.