Magnetic immobilization of Bacillus subtilis natto cells for menaquinone-7 fermentation
Magnetic immobilization of Bacillus subtilis natto cells for menaquinone-7 fermentation
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DOI：
10.1007/s00253-015-6977-3
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发表时间：
2016-01-01
影响因子：
5
通讯作者：
Berenjian, Aydin
中科院分区：
文献类型：
--
作者：
Ebrahiminezhad, Alireza;Varma, Vikas;Berenjian, Aydin
通过枯草芽孢杆菌纳豆生产甲萘醌-7（MK-7）与主要缺点相关。为了解决当前MK-7发酵面临的挑战，研究磁性纳米颗粒对细菌细胞的影响可以为强化生物过程开辟一个新的领域。本文介绍了氧化铁纳米颗粒（离子）作为MK-7工艺强化的先驱工具的新概念。在这个顺序中，离子的平均尺寸为11 nm的成功制造和其特征在于可能在原位去除目标物质从发酵培养基。制备的颗粒用于修饰和固定B。纳豆细胞与对照样品相比，氧化铁纳米颗粒的存在显著提高了MK-7的比产率（15%）。此外，制造的离子显示出从发酵培养基中原位回收细菌细胞的有希望的能力，具有超过95%的捕获效率。基于这些结果，离子可以成功地实施为MK-7生产的新工具。这项研究为磁性纳米颗粒的工业应用及其在设计强化生物过程中的未来作用提供了相当大的兴趣。
Production of menaquinone-7 (MK-7) by Bacillus subtilis natto is associated with major drawbacks. To address the current challenges in MK-7 fermentation, studying the effect of magnetic nanoparticles on the bacterial cells can open up a new domain for intensified bioprocesses. This article introduces the new concept of application of iron oxide nanoparticles (IONs) as a pioneer tool for MK-7 process intensification. In this order, IONs with the average size of 11 nm were successfully fabricated and characterized for possible in situ removal of target substances from the fermentation media. The prepared particles were used for decoration and immobilization of B. subtilis natto cells. Presence of iron oxide nanoparticles significantly enhanced the MK-7 specific yield (15 %) as compared to the control samples. In addition, fabricated IONs showed a promising ability for in situ recovery of bacterial cells from the fermentation media with more than 95 % capture efficiency. Based on the results, IONs can be implemented successfully as a novel tool for MK-7 production. This study provides a considerable interest for industrial application of magnetic nanoparticles and their future role in designing an intensified biological process.