Plasma leakage through microporous membranes. Role of phospholipids.
Plasma leakage through microporous membranes. Role of phospholipids.
复制标题
血浆通过微孔膜泄漏。
DOI：
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发表时间：
1992
期刊：
影响因子：
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通讯作者：
R. Bartlett
中科院分区：
文献类型：
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作者：
Montoya Jp;C. Shanley;S. Merz;R. Bartlett
微孔膜氧合器的血浆渗漏是长时间体外循环的一个众所周知的并发症。作者假设，双极等离子体分子，如磷脂在微孔膜上的吸附，导致在膜的疏水表面形成亲水层；这反过来又导致在正常表面张力下的等离子体泄漏。采用脂磷法测定了不同实验条件下的微孔膜氧合器纤维对磷脂的吸附。磷脂在微孔膜上的吸附具有浓度依赖性。在体外和体内都存在可重复性的血浆渗漏，渗漏的时间取决于微孔膜上吸附的磷脂的浓度。根据这些结果，作者得出结论，磷脂的吸附有助于通过微孔膜氧合器的血浆渗漏的发展。
Plasma leakage through microporous membrane oxygenators is a well known complication of prolonged extracorporeal circulation. The authors hypothesized that adsorption of bipolar plasma molecules, such as phospholipids on the microporous membrane, results in formation of a hydrophilic layer over the hydrophobic surface of the membrane; this, in turn, leads to plasma leakage at normal surface tensions. A lipid phosphorus assay was used to measure phospholipid adsorption onto the fibers of microporous membrane oxygenators tested under a variety of experimental conditions. Adsorption of phospholipids on the microporous membrane was concentration dependent. Reproducible plasma leakage occurred both in vitro and in vivo, and the time to leakage was dependent on the concentration of phospholipids adsorbed upon the microporous membrane. Based upon these results, the authors conclude that adsorption of phospholipids contributes to the development of plasma leakage through microporous membrane oxygenators.