Regulation of differentiation and proliferation of rat hepatocytes by lactose-carrying polystyrene.
Regulation of differentiation and proliferation of rat hepatocytes by lactose-carrying polystyrene.
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携带乳糖的聚苯乙烯对大鼠肝细胞分化和增殖的调节。
DOI：
10.1111/j.1525-1594.1992.tb00553.x
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发表时间：
2008
影响因子：
2.4
通讯作者：
T. Akaike
中科院分区：
文献类型：
--
作者：
A. Kobayashi;M. Goto;T. Sekine;A. Masumoto;N. Yamamoto;K. Kobayashi;T. Akaike
我们注意到肝细胞通过asialal糖蛋白受体识别低聚糖的结构，并合成了携带乳糖的苯乙烯聚合物（PVLA）作为asialal糖蛋白模型。我们发现在聚苯乙烯培养皿中培养的肝细胞分化功能和增殖可以通过改变培养皿上涂覆的PVLA量来实现。在包被大量聚乳酸的培养皿中培养的肝细胞呈圆形时，肝细胞的3h -胸腺嘧啶吸收量较低；在包被低聚乳酸的培养皿中培养的肝细胞呈圆形时，肝细胞的3h -胸腺嘧啶吸收量最高。此外，胆汁酸释放作为肝细胞的一种典型分化功能，在涂有高水平PVLA的培养皿上的肝细胞中保持较高水平。我们的研究结果表明，肝细胞分化功能和增殖的调节可以通过特异性配体介导的微观表面结构识别来实现。因此，通过设计培养基质来调节肝细胞的增殖和分化是可能的。
We noticed that hepatocytes recognize the structure of oligosaccharides via asialoglycoprotein receptors and synthesized lactose-carrying styrene polymer (PVLA) as an asialoglycoprotein model. We found that the regulation of differentiated function and proliferation of hepatocytes that were cultured on polystyrene dishes can be achieved by varying the amount of PVLA coated on the dishes. The 3H-thymidine uptake of cultured hepatocytes is low for cells cultured on dishes coated with high amounts of PVLA in which the hepatocytes are round and is highest for those cultured on dishes with a low PVLA coating level in which the hepatocytes are spread in shape. Moreover, bile acid release as a typical differentiated function of hepatocytes was maintained at higher levels in hepatocytes attached on dishes coated with a high level of PVLA. Our results indicated that regulation of differentiated function and proliferation in hepatocytes could be achieved by specific ligand-mediated recognition of microscopic surface structure. Thus, it may be possible to regulate proliferation and differentiation of hepatocytes by design of the culturing substrata.