Osteoclast fusion is based on heterogeneity between fusion partners.
Osteoclast fusion is based on heterogeneity between fusion partners.
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DOI：
10.1007/s00223-014-9864-5
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发表时间：
2014-07
影响因子：
4.2
通讯作者：
Soe, Kent
中科院分区：
文献类型：
--
作者：
Hobolt-Pedersen, Anne-Sofie;Delaisse, Jean-Marie;Soe, Kent
骨吸收破骨细胞通过单核前体融合形成。他们在融合过程中选择的合作伙伴仍然不清楚。我们假设破骨细胞在选择融合伴侣时具有选择性，这种选择性是基于细胞成熟阶段、融合因子表达和细胞组织的异质性。破骨细胞融合因子CD 47、树突状细胞特异性跨膜蛋白（DC-STAMP）和合胞素-1的免疫荧光染色支持了这一假设。这些染色揭示了所有三个因素在破骨细胞的给定文化的异质性定位模式。CD 47主要定位于小破骨细胞和前破骨细胞，DC-STAMP表达阳性，但组织蛋白酶K表达阴性。CD 47在早期破骨细胞融合步骤中的作用也从使用CD 47阻断抗体的实验中提出，其导致小破骨细胞融合的抑制。相反，连接蛋白43的阻断影响了具有四个或更多核的较大破骨细胞的融合。不同的融合因子在破骨细胞融合的不同阶段发挥作用的建议支持了破骨细胞群体异质性的观点;我们的研究结果表明，破骨细胞融合确实是基于异质性的。考虑到破骨细胞发育和融合的体内环境，我们的研究结果似乎非常适用，并为骨和融合研究中的关键问题提供了新的重要见解。
Bone-resorbing osteoclasts are formed through fusion of mononucleated precursors. Their choice of partners during the fusion process remains unclear. We hypothesized that osteoclasts are selective in their choice of fusion partner and that this selectivity is based on heterogeneity among the cells with respect to their maturation stage and their expression and cellular organization of fusion factors. Support for this hypothesis was found from immunofluorescence staining of the osteoclast fusion factors CD47, dendritic cell-specific transmembrane protein (DC-STAMP), and syncytin-1. These stainings revealed heterogeneous localization patterns of all three factors within a given culture of osteoclasts. CD47 was found to be localized primarily in small osteoclasts and preosteoclasts, which were also positive for DC-STAMP but negative for cathepsin K expression. A role of CD47 in the early osteoclast fusion steps was also suggested from experiments with a CD47 blocking antibody, which resulted in an inhibition of the fusion of small osteoclasts. Conversely, blocking of connexin 43 affected the fusion of larger osteoclasts with four or more nuclei. The suggestion that different fusion factors function at different stages of osteoclast fusion supports the idea of heterogeneity in the osteoclast population; our results suggest that osteoclast fusion is indeed based on heterogeneity. Considering the in vivo environment in which osteoclasts develop and fuse, our findings seem very applicable and provide novel, important insight into key issues in bone and fusion research.
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影响因子：
6.2
作者：
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DOI：
10.1016/j.bbrc.2005.04.032
发表时间：
2005-06-17
影响因子：
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作者：
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