Distinct octopamine cell population residing in the CNS abdominal ganglion controls ovulation in Drosophila melanogaster
Distinct octopamine cell population residing in the CNS abdominal ganglion controls ovulation in Drosophila melanogaster
复制标题
DOI：
10.1016/j.ydbio.2003.07.019
复制
发表时间：
2003-12-01
影响因子：
2.7
通讯作者：
Monastirioti, M
中科院分区：
文献类型：
--
作者：
Monastirioti, M
章鱼胺是一种重要的神经活性物质，调节无脊椎动物的多种生理功能和行为。其生物合成包括两个步骤，其中一个步骤由酪胺β-羟化酶（TBH）催化。Tbetah基因先前已从黑腹果蝇中克隆，并且已经产生无效突变，导致显示出严重雌性不育的无章鱼胺果蝇。在这里，我表明，排卵过程是有缺陷的突变体女性导致卵巢内的成熟卵母细胞堵塞。通过在成年雌性中表达在hsp 70启动子控制下的Tbetah cDNA，有条件地拯救表型。当TBH通过GAL 4-UAS系统在表达TBH并产生章鱼胺的CNS腹神经节细胞中表达时，突变雌性的生育力也恢复。这种神经元群体不同于多巴胺和多巴胺表达细胞，表明该区域中三种物质的表达和功能的不同模式。最后，我证明，这些TBH表达细胞项目的外围，他们支配的卵巢和输卵管的生殖系统。上述结果指出，在女性生殖系统的特定部位，神经元可以合成和释放章鱼胺，而这些部位需要胺来触发排卵。(C)2003年爱思唯尔公司All rights reserved.
Octopamine is an important neuroactive substance that modulates several physiological functions and behaviors of invertebrate species. Its biosynthesis involves two steps, one of which is catalyzed by Tyramine beta-hydroxylase enzyme (TBH). The Tbetah gene has been previously cloned from Drosophila melanogaster, and null mutations have been generated resulting in octopamine-less flies that show profound female sterility. Here, I show that ovulation process is defective in the mutant females resulting in blockage of mature oocytes within the ovaries. The phenotype is conditionally rescued by expressing a Tbetah cDNA under the control of a hsp70 promoter in adult females. Fertility of the mutant females is also restored when TBH is expressed, via the GAL4-UAS system, in cells of the CNS abdominal ganglion that express TBH and produce octopamine. This neuronal population differs from the dopamine- and serotonin-expressing cells indicating distinct patterns of expression and function of the three substances in the region. Finally, I demonstrate that these TBH-expressing cells project to the periphery where they innervate the ovaries and the oviducts of the reproductive system. The above results point to a neuronal focus that can synthesize and release octopamine in specific sites of the female reproductive system where the amine is required to trigger ovulation. (C) 2003 Elsevier Inc. All rights reserved.