Lipopolysaccharide derived from the digestive tract activates inflammatory gene expression and inhibits casein synthesis in the mammary glands of lactating dairy cows.
Lipopolysaccharide derived from the digestive tract activates inflammatory gene expression and inhibits casein synthesis in the mammary glands of lactating dairy cows.
复制标题
来自消化道的脂多糖可激活泌乳奶牛乳腺中的炎症基因表达并抑制酪蛋白合成。
DOI：
10.18632/oncotarget.7371
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发表时间：
2016-03-01
期刊：
影响因子：
--
通讯作者：
Shen X
中科院分区：
文献类型：
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作者：
Zhang K;Chang G;Xu T;Xu L;Guo J;Jin D;Shen X
为了满足哺乳期的营养需求，奶牛通常饲喂高精料日粮(HC)。然而，高谷物饲喂会导致亚急性瘤胃酸中毒(SARA)，这是一种导致牛奶蛋白质抑制的代谢紊乱。本研究旨在研究瘤胃内释放的脂多糖(LPS)对饲喂HC日粮的哺乳期奶牛乳腺炎性基因表达和酪蛋白合成的影响。我们发现，在喂养15周后，HC组的乳蛋白显著降低。总体而言，HC组瘤胃液、乳动、静脉内毒素浓度升高。用转录组芯片检测乳腺信号通路的改变。在HC组，参与炎症反应的信号通路被激活，而参与蛋白质合成的信号通路被抑制。HC组炎症反应相关基因TLR4、NF-кB和促炎基因表达增加，而αS1-酪蛋白(CSN1S1)、β-酪蛋白(CSN2)、mTOR和S6K基因表达降低。此外，蛋白质的表达与相应的基因表达一致。饲喂高脂饲料后，饲喂高脂饲料的哺乳期奶牛，瘤胃内的脂多糖增加了乳腺炎症基因的表达，抑制了酪蛋白的合成。
To meet the nutrition requirements of lactation, dairy cows are usually fed a high concentrate diet (HC). However, high-grain feeding causes subacute ruminal acidosis (SARA), a metabolic disorder that causes milk protein depression. This study aimed to investigate the effect of lipopolysaccharide (LPS) released in the rumen on inflammatory gene expression and casein synthesis in mammary glands of lactating dairy cows fed a HC diet. We found that milk protein was significantly decreased in the HC group after 15 weeks of feeding. Overall, LPS concentrations in the rumen fluid, lacteal artery and vein were increased in the HC group. Transcriptome microarray was used to evaluate alterations in the signaling pathway in mammary glands. Signaling pathways involved in inflammatory responses were activated, whereas those involved in protein synthesis were inhibited in the HC group. mRNA expression involved in inflammatory responses, including that of TLR4, NF-кB and pro-inflammatory genes, was increased in the HC group, while αs1-casein (CSN1S1), β-casein (CSN2), mTOR and S6K gene expression were decreased. Moreover, protein expression was consistent with the corresponding gene expression. After feeding with an HC diet, LPS derived from the rumen increased inflammatory gene expression and inhibited casein synthesis in the mammary glands of lactating dairy cows fed a HC diet.