Inhibition of lens epithelial cell growth by induction of apoptosis: potential for prevention of posterior capsule opacification.
Inhibition of lens epithelial cell growth by induction of apoptosis: potential for prevention of posterior capsule opacification.
复制标题
通过诱导细胞凋亡抑制晶状体上皮细胞生长：预防后囊混浊的潜力。
DOI：
10.1089/10807680152729275
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
James,ER
中科院分区：
文献类型：
--
作者：
Geissler,FT;Li,DW;James,ER
以人和兔晶状体上皮细胞(LEC)和兔晶状体上皮细胞株(N/N1003A)为研究对象，在含10%胎牛血清的Dulbecco最低基本培养液(MEM)中培养人和兔晶状体上皮细胞。采用台盼蓝染色、~3H-胸腺嘧啶核苷掺入法、Annexin/碘化丙啶染色、钙黄素AM/溴化乙锭染色和DNA梯带法检测LEC的存活率和细胞凋亡率。人的囊膜切开术标本同样暴露于钙霉素，并用钙黄素AM/溴化乙锭染色检测细胞凋亡。钙霉素可诱导兔晶状体上皮细胞和人晶状体上皮细胞发生凋亡，并可在30分钟内检测到导致细胞凋亡的变化。人和兔晶状体上皮细胞活力和生长的下降呈剂量依赖性。这些数据支持进一步评价诱导细胞凋亡作为预防人类初级白内障手术后后发性白内障的一种可能的治疗机制。
As a model of the cell proliferation occurring in posterior capsule opacification (PCO), lens epithelial cells (LEC) from human and rabbit capsulotomies, and a rabbit LEC line (N/N1003A) were grown in Dulbecco's Minimal Essential Media (MEM) with 10% fetal calf serum. LEC were exposed to the calcium ionophore, calcimycin, and viability was assessed by trypan blue staining, growth by3H-thymidine incorporation and apoptosis by annexin/propidium iodide staining, calcein AM/ethidium bromide staining and DNA laddering. Human capsulotomy samples were similarly exposed to calcimycin, and apoptosis assayed by calcein AM/ethidium bromide staining. Calcimycin exposure induced apoptosis in both rabbit LEC cultures and human LEC, and changes leading to apoptosis could be detected within 30 minutes of calcimycin treatment. The decrease in viability and growth in human and rabbit LEC was dose-dependent. These data support the further evaluation of apoptosis induction as a possible treatment mechanism to prevent development of PCO following primary cataract surgery in humans.