TRPA1 contributes to cold, mechanical, and chemical Nociception but is not essential for hair-cell transduction
TRPA1 contributes to cold, mechanical, and chemical Nociception but is not essential for hair-cell transduction
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DOI：
10.1016/j.neuron.2006.03.042
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发表时间：
2006-04-20
期刊：
影响因子：
16.2
通讯作者：
Corey, DP
中科院分区：
文献类型：
--
作者：
Kwan, KY;Allchorne, AJ;Corey, DP
TRPA1是离子通道的瞬时受体电位（TRP）家族的成员，由背根神经节神经元和内耳细胞表达，在那里它被提出在感知声音、疼痛的寒冷和刺激性化学物质中的作用。为了测试TRPA1的体内作用，我们产生了一种小鼠，其中Trpa1基因的正常功能所需的必需外显子被删除。敲除小鼠在对芥子油、寒冷（类似于0摄氏度）和点状机械刺激的反应中表现出行为缺陷。这些小鼠对噪音有正常的惊吓反射，正常的平衡感，正常的听觉脑干反应，前庭毛细胞中的正常转导电流。TRPA1显然不是毛细胞转导所必需的，但有助于伤害感受器感觉神经元中机械、冷和化学刺激的转导。
TRPA1, a member of the transient receptor potential (TRP) family of ion channels, is expressed by dorsal root ganglion neurons and by cells of the inner ear, where it has proposed roles in sensing sound, painful cold, and irritating chemicals. To test the in vivo roles of TRPA1, we generated a mouse in which the essential exons required for proper function of the Trpa1 gene were deleted. Knockout mice display behavioral deficits in response to mustard oil, to cold (similar to 0 degrees C), and to punctate mechanical stimuli. These mice have a normal startle reflex to loud noise, a normal sense of balance, a normal auditory brainstem response, and normal transduction currents in vestibular hair cells. TRPA1 is apparently not essential for hair-cell transduction but contributes to the transduction of mechanical, cold, and chemical stimuli in nociceptor sensory neurons.