Metal cation toxicity in the alga Gracilaria domingensis as evaluated by the daily growth rates in synthetic seawater
Metal cation toxicity in the alga Gracilaria domingensis as evaluated by the daily growth rates in synthetic seawater
复制标题
DOI：
10.1007/s10811-013-0036-1
复制
发表时间：
2013-04
影响因子：
3.3
通讯作者：
L. Mendes;L. Zambotti‐Villela;P. Colepicolo;E. Marinho-Soriano;Cassius Vinicius Stevani;N. Yokoya
中科院分区：
文献类型：
--
作者：
L. Mendes;L. Zambotti‐Villela;P. Colepicolo;E. Marinho-Soriano;Cassius Vinicius Stevani;N. Yokoya
江蓠属的大型藻类作为琼脂生产的原料具有相当大的经济重要性，并且属于耐受高浓度金属的重要生物群体。通过测量新鲜质量变化（即，本文报道了48小时水生毒性试验期间，江蓠（Gracilaria domingensis）的日生长率。该实验室将该生物暴露于14种不同的金属阳离子以及人工合成海水中的重金属阴离子。分别通过电感耦合等离子体原子发射光谱法和环境保护局推荐的软件MINTEQA 2测定这些离子物质的实际浓度（在IC 50值下）和游离离子（含水络合物）的比例。根据游离IC 50值（IC 50 F），离子的毒性排序为：Cd 2 +<$Cu 2 +<$Pb 2 +<$Zn 2 +<$Ni 2 +> Co 2 +> La 3 +<$Mn 2 +> Ca 2 +~ Li+<$MoO 42 − <$Sr 2 +> Mg 2 +<$K+> Na+。作为海洋食物链中第一营养级的一员，G. domingensisis是一种适当的目标生物，既可用于发展毒理学分析，也可作为海洋退化的生物指标。
Macroalgae of the genusGracilariahave considerable economic importance as raw material for agar production and belong to an important group of organisms that are tolerant of high concentrations of metal. The median inhibitory concentration (IC50) values obtained by measuring the ratio of fresh mass variation (i.e., daily growth rates) of the red macroalgaGracilaria domingensisduring a 48-h aquatic toxicity assay are reported here. The alga was exposed to 14 different metal cations as well as the molybdate anion in synthetic seawater. The actual concentrations of these ionic species (at IC50values) and the proportion of free ions (aqueous complexes) were determined by inductively coupled plasma atomic emission spectroscopy and the Environmental Protection Agency-recommended software, MINTEQA2, respectively. Based on the free IC50values (IC50F), the ions were ranked in terms of toxicity: Cd2+≫ Cu2+≫ Pb2+≫ Zn2+≫ Ni2+> Co2+> La3+≫ Mn2+> Ca2+~ Li+≫ MoO42−≫ Sr2+> Mg2+≫ K+> Na+. As a member of the first trophic level in the marine food chain,G. domingensisis an appropriate target organism both for the development of toxicological assays and as a bioindicator of marine degradation.