Distribution of RA175/TSLC1/SynCAM, a member of the immunoglobulin superfamily, in the developing nervous system
Distribution of RA175/TSLC1/SynCAM, a member of the immunoglobulin superfamily, in the developing nervous system
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DOI：
10.1016/j.devbrainres.2004.10.015
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发表时间：
2005-02-08
期刊：
影响因子：
--
通讯作者：
Momoi, T
中科院分区：
文献类型：
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作者：
Fujita, E;Urase, K;Momoi, T
RA175是具有反式相互作用活性的免疫球蛋白超家族的新成员，它作为肺癌的肿瘤抑制因子(TSLC1)和促进功能性突触形成的细胞黏附分子(SynCAM)发挥作用。R-A175/TSLC1/SynCAM神经网络形成在神经发生过程中的生物学功能还知之甚少。我们研究了RA175/TSLC1/SynCAM蛋白与免疫球蛋白超家族的其他成员如NCAM、L1和Tag-1在小鼠神经系统发育中的分布和共存。该蛋白定位于胚胎9.5天的脑神经上皮细胞、胚胎10.5天的神经峰、胚胎10.5天的运动神经元和胚胎16.5天的嗅觉上皮细胞，与RA175/TSLC1/SynCAM的表达一致。在E10.5-11.5脊髓的底板、腹根和背索的束状轴突上表达较强，并与NCAM和L1共存于腹根和背索，与Tag-I部分共存于连合轴突和背扇。在E13.5-15.5脑内，RA175/TSLC1/SynCAM与NCAM和L1共同定位于发育中的内囊丘脑皮质纤维，部分与Tag-1共同定位于中间带皮质传出轴突。RA175/TSLC1/SynCAM定位于体外培养的部分皮质神经元的轴突上。因此，除了神经上皮和突触的细胞黏附活性外，RA175/TSLC1/SynCAM可能还参与神经元的迁移、轴突生长、寻路和分化神经元轴突上的分束。(C)2004爱思唯尔B.V.保留所有权利。
RA175 is a new member of the immunoglobulin superfamily with trans interaction activity, and it plays a role as a tumor suppressor in lung carcinoma (TSLC1) and as a cell adhesion molecule promoting the formation of functional synapses (SynCAM). Little is known about the biological function of R-A175/TSLC1/SynCAM neural network formation during neurogenesis. We examined the distribution and colocalization of the RA175/TSLC1/SynCAM protein with other members of the immunoglobulin superfamily such as NCAM, L1, and TAG-1 in the mouse developing nervous system. Consistent with the expression of RA175/TSLC1/SynCAM mRNA, the protein was localized in the brain neuroepithelium at embryonic day (E) 9.5, neural crest at E10.5, motor neurons at E10.5, and olfactory epithelium at E16.5. In contrast with its mRNA, the protein was intensely detected on the fasciculated axons in the floor plates, ventral root, and dorsal funiculus in the E10.5-11.5 spinal cord and colocalized with NCAM and L1 on the ventral root and dorsal funiculus and partly colocalized with TAG-I on the commissural axons and dorsal faniculus. In the E13.5-15.5 brain, RA175/TSLC1/SynCAM colocalized with NCAM and L1 on the developing thalamocortical fibers from the internal capsule (IC) and partly colocalized with TAG-1 on the cortical efferent axons in the intermediate zone (IZ). RA175/TSLC1/SynCAM was localized on the axons of some of the cortical neurons cultured in vitro. Thus, in addition to cell adhesion activity in the neuroepithelium and the synapses, RA175/TSLC1/SynCAM may be involved in neuronal migration, axon growth, pathfinding, and fasciculation on the axons of differentiating neurons. (C) 2004 Elsevier B.V. All rights reserved.