Efficient replication systems for hepatitis C virus using a new human hepatoma cell line
Efficient replication systems for hepatitis C virus using a new human hepatoma cell line
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DOI：
10.1016/j.virusres.2009.08.006
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发表时间：
2009-12-01
期刊：
影响因子：
5
通讯作者：
Ikeda, Masanori
中科院分区：
文献类型：
--
作者：
Kato, Nobuyuki;Mori, Kyoko;Ikeda, Masanori
持续性丙型肝炎病毒（HCV）感染导致慢性肝病，是一个严重的全球性健康问题。基于细胞培养的HCV RNA持续复制系统和感染性HCV生产系统广泛应用于HCV研究。然而，持续的HCV生产系统已开发仅用于HuH-7肝癌细胞。在这里，我们发现了一个新的人肝癌细胞系，Li 23，使持续的HCV生产和抗HCV试剂检测。Li 23的cDNA表达谱与HuH-7的不同，尽管这两种细胞具有相似的肝脏特异性表达谱。我们使用具有适应性突变的特定组合的HCV RNA来开发HCV复制子系统和基因组长度的HCV RNA复制系统，包括报告基因测定系统。最后，Li 23来源的细胞持续产生HCV株的感染性病毒。Li 23衍生的细胞对于理解HCV生命周期和寻找抗病毒靶点是潜在有用的。(C)2009 Elsevier B. V.保留所有权利。
Persistent hepatitis C virus (HCV) infection causes chronic liver diseases and is a serious global health problem. Cell culture-based persistent HCV RNA replication systems and infectious HCV production systems are widely used in HCV research. However, persistent HCV production systems have been developed only for HuH-7 hepatoma cells. Here we found a new human hepatoma cell line, Li23, that enables persistent HCV production and anti-HCV reagent assay. Li23's cDNA expression profile differed from HuH-7's, although the two cells had similar liver-specific expression profiles. We used HCV RNA with a specific combination of adaptive mutations to develop an HCV replicon system and genome-length HCV RNA replicating systems including a reporter assay system. Finally, Li23-derived cells persistently produced infectious virus of an HCV strain. Li23-derived cells are potentially useful for understanding the HCV life cycle and for finding antiviral targets. (C) 2009 Elsevier B.V. All rights reserved.