E-cigarette vapour enhances pneumococcal adherence to airway epithelial cells
E-cigarette vapour enhances pneumococcal adherence to airway epithelial cells
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DOI：
10.1183/13993003.01592-2017
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发表时间：
2018-02-01
影响因子：
24.3
通讯作者：
Grigg, Jonathan
中科院分区：
文献类型：
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作者：
Miyashita, Lisa;Suri, Reetika;Grigg, Jonathan
电子烟蒸汽含有自由基，有可能引起氧化应激。由于气道细胞中的氧化应激增加了血小板活化因子受体（PAFR）的表达，并且PAFR被肺炎球菌吸收粘附到宿主细胞上，我们假设电子烟蒸气增加了肺炎球菌对气道细胞的粘附。在非吸烟对照组和吸烟5分钟前后的成人中评估了鼻上皮PAFR。我们确定了蒸汽对氧化应激诱导的PAFR依赖性肺炎球菌粘附到体外气道上皮细胞的影响，以及对小鼠鼻咽部肺炎球菌定植的影响。通过质谱法对蒸汽进行元素分析，并通过体外抗氧化剂消耗评估蒸汽的氧化电位。在基线鼻上皮PAFR表达方面，蒸汽使用者（n=11）和对照组（n=6）之间没有差异。Vaping增加鼻PAFR表达。含尼古丁和不含尼古丁的电子烟蒸汽增加了体外肺炎球菌对气道细胞的粘附。PAFR阻断剂CV 3988可减弱体外蒸汽刺激的粘附。含尼古丁的电子烟蒸汽增加了小鼠鼻PAFR表达和鼻咽肺炎球菌定植。蒸汽含有氧化还原活性金属，具有相当大的氧化活性，抗氧化剂N-乙酰半胱氨酸减弱了粘附力。这项研究表明，电子烟蒸汽有可能增加对肺炎球菌感染的易感性。
E-cigarette vapour contains free radicals with the potential to induce oxidative stress. Since oxidative stress in airway cells increases platelet-activating factor receptor (PAFR) expression, and PAFR is co-opted by pneumococci to adhere to host cells, we hypothesised that E-cigarette vapour increases pneumococcal adhesion to airway cells.Nasal epithelial PAFR was assessed in non-vaping controls, and in adults before and after 5 min of vaping. We determined the effect of vapour on oxidative stress-induced, PAFR-dependent pneumococcal adhesion to airway epithelial cells in vitro, and on pneumococcal colonisation in the mouse nasopharynx. Elemental analysis of vapour was done by mass spectrometry, and oxidative potential of vapour assessed by antioxidant depletion in vitro.There was no difference in baseline nasal epithelial PAFR expression between vapers (n=11) and controls (n=6). Vaping increased nasal PAFR expression. Nicotine-containing and nicotine-free E-cigarette vapour increased pneumococcal adhesion to airway cells in vitro. Vapour-stimulated adhesion in vitro was attenuated by the PAFR blocker CV3988. Nicotine-containing E-cigarette vapour increased mouse nasal PAFR expression, and nasopharyngeal pneumococcal colonisation. Vapour contained redox-active metals, had considerable oxidative activity, and adhesion was attenuated by the antioxidant N-acetyl cysteine.This study suggests that E-cigarette vapour has the potential to increase susceptibility to pneumococcal infection.