Magnetic resonance imaging of the pancreas and pancreatic tumors in a mouse orthotopic model of human cancer
Magnetic resonance imaging of the pancreas and pancreatic tumors in a mouse orthotopic model of human cancer
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DOI：
10.1002/ijc.11281
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发表时间：
2003-09-20
影响因子：
6.4
通讯作者：
Moore, A
中科院分区：
文献类型：
--
作者：
Grimm, J;Potthast, A;Moore, A
胰腺癌的发病率不断上升，生存率极低。为了开发新的治疗策略，对胰腺癌的动物模型进行了广泛的研究。原位胰腺肿瘤模型，其中肿瘤被植入胰腺，比皮下肿瘤模型更接近人类疾病，但难以监测。在我们的研究中，我们报告了一种磁共振成像（MRI）的方法来可视化小鼠胰腺和监测原位植入胰腺肿瘤。使用MRI扫描仪对正常小鼠胰腺和植入人胰腺癌细胞系的小鼠胰腺进行成像。钆（Gd）-DTPA增强的T1和T2加权标准序列用于识别胰腺和监测原位肿瘤生长在30天内的目标。使用MRI可以很容易地识别胰腺以及植入的肿瘤。在T2加权像上，植入的肿瘤在植入侧容易被可视化，具有高信号强度。应用造影剂后，肿瘤显示出增强。肿瘤内的异质性可以描绘，对应于组织学，肿瘤的大小可以精确测量。MR作为一种非侵入性的高分辨率图像模式来监测小鼠胰腺以及原位胰腺肿瘤的大小，生长甚至异质性区域。(C)2003 Wiley-Liss，Inc.
Pancreatic adenocarcinoma has a rising incidence and a very poor survival rate. To develop new treatment strategies, extensive research is performed on animal models of pancreatic cancer. Orthotopic pancreatic tumors models, where the tumor is implanted into the pancreas, resemble the human disease more closely than subcutaneous tumor models, yet are difficult to monitor. In our study we report a magnetic resonance imaging (MRI) approach to visualize the pancreas in mice and to monitor orthotopically implanted pancreatic tumors. An MRI scanner was used to image normal murine pancreas and the pancreas of mice implanted with a human pancreatic adenocarcinoma cell line. Gadolinium (Gd)-DTPA-enhanced T1 - and T2-weighted standard sequences were used with the objective to identify the pancreas and to monitor the growth of orthotopic tumors during 30 days. The pancreas as well as the implanted tumors could be easily identified using MRI. On T2-weighted images, the implanted tumors were easily visualized at the implantation side with high signal intensity. After application of a contrast agent, the tumors showed an enhancement. Heterogeneities within the tumor could be delineated, corresponding to histology, and the size of the tumor could be measured precisely. MR serves as a noninvasive high-resolution image modality to monitor murine pancreas as well as size, growth and even areas of heterogeneity in orthotopic pancreatic tumors. (C) 2003 Wiley-Liss, Inc.