Tetrandrine, a potent antifungal agent: inhibits mycelial growth and virulence of Botrytis cinerea.
Tetrandrine, a potent antifungal agent: inhibits mycelial growth and virulence of Botrytis cinerea.
复制标题
粉防己碱，一种有效的抗真菌剂：抑制灰霉病菌的菌丝生长和毒力。
DOI：
10.1094/phyto-10-20-0446-r
复制
发表时间：
2020-12
期刊：
影响因子：
3.2
通讯作者：
Li D
中科院分区：
文献类型：
--
作者：
Li P;Zou J;董妍涵;Jiang J;Liang W;Li D
粉防己碱 (TET) 是一种有效的钙通道阻滞剂，用于治疗高血压和炎症。目前，TET主要用于治疗多种人类疾病，而关于使用TET对抗植物病原体的信息很少。在这项研究中，我们探讨了 TET 对植物病原体灰葡萄孢 (Botrytis cinerea) 的抗真菌活性。我们发现，施用低浓度的 TET 可以有效抑制马铃薯葡萄糖琼脂糖上生长的真菌的菌丝生长，并降低番茄植株中灰霉病菌的毒力。实时PCR显示，在TET存在下，药物外排泵相关基因（乙醇脱氢酶1、多药/信息素输出蛋白、多效性耐药蛋白1和突触小泡转运蛋白）的表达下调。最后，我们证明 TET 与异菌脲具有协同作用，导致体外和体内对灰霉病菌的抑制增加。这些结果表明 TET 可能作为减少灰霉病的有效抗真菌剂。
Tetrandrine (TET) is a potent calcium channel blocker used for the treatment of hypertension and inflammation. Currently, TET is predominantly used to treat a variety of human diseases, and there is little information regarding the use of TET against plant pathogens. In this study, we explored the antifungal activity of TET on a plant pathogen, Botrytis cinerea. We show that administration of low concentrations of TET effectively inhibited hyphal growth of fungus grown on potato dextrose agarose, and decreased the virulence of B. cinerea in tomato plants. Real-time PCR revealed that the expression of drug efflux pump related genes (alcohol dehydrogenase 1, multi-drug/pheromone exporter, pleiotropic drug resistance protein 1, and synaptic vesicle transporter) were down-regulated in the presence of TET. Finally, we show that TET acts synergistically with iprodione, resulting in increased inhibition of B. cinerea both in vitro and in vivo. These results indicate that TET might act as an effective antifungal agent in reducing grey mold disease.