Flow cytometric characterizations of leukocyte subpopulations in the peripheral blood of northern pig-tailed macaques (Macaca leonina).
Flow cytometric characterizations of leukocyte subpopulations in the peripheral blood of northern pig-tailed macaques (Macaca leonina).
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DOI：
10.13918/j.issn.2095-8137.2014.6.465
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发表时间：
2014-11
期刊：
影响因子：
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通讯作者：
Hong-yi Zheng;Ming-Xu Zhang;Lin-Tao Zhang;Xiao-liang Zhang;W. Pang;Longbao Lyu;Yong-tang Zheng
中科院分区：
文献类型：
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作者：
Hong-yi Zheng;Ming-Xu Zhang;Lin-Tao Zhang;Xiao-liang Zhang;W. Pang;Longbao Lyu;Yong-tang Zheng
猪尾猕猴（Macaca nemistrina group）近年来被广泛用作各种人类疾病的非人灵长类动物模型，特别是由于其对HIV-1的敏感性而用于AIDS研究。北方猪尾猕猴（M. leonina）分布于中国及周边东南亚国家。尽管从2012年开始，北方猪尾猕猴作为实验动物被大规模饲养，但白细胞正常水平的参考值并不存在。为了获得这些信息，从不同年龄的雄性和雌性健康北方猪尾猕猴中收集了62份血液样品。采用流式细胞仪检测T淋巴细胞、B淋巴细胞、自然杀伤（NK）细胞、单核细胞等主要白细胞亚群的正常范围，以及淋巴细胞表面活化或分化相关分子（CD 38、HLA-DR、CCR 5、CD 21、IgD、CD 80和CD 86）的表达水平。B细胞计数随年龄增长而下降，而CD 8（+）T细胞、NK细胞及CD 38（+）HLA-DR（+）CD 4（+）T细胞频率与年龄呈正相关。除CD 4（+）T细胞外，男性白细胞亚群计数均高于女性。雄性动物的B细胞中IgD和CD 21的表达水平也较高。本研究提供了北方短尾猴白细胞亚群的基本资料，并与我国常用的恒河猴（M. mulatta），这对北方猪尾猕猴的生物医学应用具有重要意义。
Pig-tailed macaques (Macaca nemistrina group) have been extensively used as non-human primate animal models for various human diseases in recent years, notably for AIDS research due to their sensitivity to HIV-1. Northern pig-tailed macaques (M. leonina) are distributed in China and other surrounding Southeast Asia countries. Although northern pig-tailed macaques have been bred on a large scale as experimental animals since 2012, the reference value of normal levels of leukocytes is not available. To obtain such information, 62 blood samples from male and female healthy northern pig-tailed macaques at different ages were collected. The normal range of major leukocyte subpopulations, such as T lymphocytes, B lymphocytes, natural killer (NK) cells, monocytes, and the expression levels of activation or differentiation related molecules (CD38, HLA-DR, CCR5, CD21, IgD, CD80 and CD86) on lymphocytes were analyzed by flow cytometry. The counts of B cells decreased with age, but those of CD8(+) T cells and NK cells and the frequency of CD38(+)HLA-DR(+)CD4(+) T cells were positively correlated with age. The counts of leukocyte subpopulations were higher in males than those in females except for CD4(+) T cells. Males also showed higher expression levels of IgD and CD21 within B cells. This study provides basic data about the leukocyte subpopulations of northern pig-tailed macaques and compares this species with commonly used Chinese rhesus macaques (M. mulatta), which is meaningful for the biomedical application of northern pig-tailed macaques.