Evaluation of the antibacterial activity of the methylene chloride extract of Miconia ligustroides, isolated triterpene acids, and ursolic acid derivatives
Evaluation of the antibacterial activity of the methylene chloride extract of Miconia ligustroides, isolated triterpene acids, and ursolic acid derivatives
复制标题
DOI：
10.3109/13880200903062648
复制
发表时间：
2010-02-01
影响因子：
3.8
通讯作者：
da Silva Filho, Ademar A.
中科院分区：
文献类型：
--
作者：
Cunha, Wilson R.;de Matos, Geilton X.;da Silva Filho, Ademar A.
对 Miconia ligustroides (DC.) Naudin（野牡丹科）的二氯甲烷提取物、分离的化合物熊果酸和齐墩果酸以及这些酸的混合物以及熊果酸衍生物进行了针对以下微生物的评估：蜡状芽孢杆菌 (ATCC 14579)、霍乱弧菌 (ATCC 9458)、沙门氏菌 猪霍乱 (ATCC 10708)、肺炎克雷伯菌 (ATCC 10031) 和肺炎链球菌 (ATCC 6305)。抗菌活性评价时采用微量稀释法测定最低抑菌浓度(MIC)。二氯甲烷提取物对选定的微生物没有显示出活性。熊果酸对蜡状芽孢杆菌有活性，MIC值为20μg/mL。齐墩果酸对蜡状芽孢杆菌和肺炎链球菌有效，两种情况下的 MIC 均为 80 μg/mL。三萜、熊果酸和齐墩果酸的混合物不会增强抗菌活性。然而，由熊果酸制备的乙酰基和甲酯衍生物增加了对肺炎链球菌的抑制活性。
The methylene chloride extract of Miconia ligustroides (DC.) Naudin (Melastomataceae), the isolated compounds ursolic and oleanolic acids and a mixture of these acids, and ursolic acid derivatives were evaluated against the following microorganisms: Bacillus cereus (ATCC 14579), Vibrio cholerae (ATCC 9458), Salmonella choleraesuis (ATCC 10708), Klebsiella pneumoniae (ATCC 10031), and Streptococcus pneumoniae (ATCC 6305). The microdilution method was used for determination of the minimum inhibitory concentration (MIC) during evaluation of the antibacterial activity. The methylene chloride extract showed no activity against the selected microorganisms. Ursolic acid was active against B. cereus, showing a MIC value of 20 mu g/mL. Oleanolic acid was effective against B. cereus and S. pneumoniae with a MIC of 80 mu g/mL in both cases. The mixture of triterpenes, ursolic and oleanolic acids, did not enhance the antimicrobial activity. However, the acetyl and methyl ester derivatives, prepared from ursolic acid, increased the inhibitory activity for S. pneumoniae.