LncRNA7467 participated in shell biomineralization in pearl oyster Pinctada fucata martensii
LncRNA7467 participated in shell biomineralization in pearl oyster Pinctada fucata martensii
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DOI：
10.1016/j.aqrep.2022.101398
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发表时间：
2022-11-03
影响因子：
3.7
通讯作者：
He，Maoxian
中科院分区：
文献类型：
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作者：
Zhang，Hua;Yao，Gaoyou;He，Maoxian
长链非编码RNA（longnoncodingRNA，LncRNA）在多种生物学过程中发挥着重要作用，包括表观遗传调控、转录调控和转录后调控。然而，目前对lncRNA的研究主要局限于哺乳动物，而对双壳类lncRNA功能的研究还很缺乏。本研究从合浦珠母贝（Pinctada fucata martensii）中克隆到一个新的lncRNA，命名为lncRNA 7467，位于第9号染色体14962920-14963218 bp。有趣的是，lncRNA 7467在所测试的组织中的外套膜组织中显示出最高的表达水平，并且响应于受损壳的修复和再生。注射lncRNA 7467特异性双链RNA敲低基因表达后，棱柱层内表面发生明显变化，N44基因相应受到抑制。另外，lncRNA 7467主要分布在细胞质中，可能主要在转录后调控中发挥作用。这些工作表明lncRNA 7467参与了珍珠牡蛎的生物矿化过程。F. martensii。
Long noncoding RNAs (LncRNAs) are reported to have multiple roles in various biological processes, including epigenetic regulation, transcriptional regulation and post-transcriptional regulation. However, the current studies for lncRNAs are mainly limited to mammalian species, and the investigations of functional roles for lncRNAs in bivalve species are still lacking. Here, we identified a novel lncRNA namedlncRNA7467from pearl oysterPinctada fucata martensii, located in chromosome 9 at 14962920–14963218 bp. Intriguingly,lncRNA7467showed the highest expression level in mantle tissue among tested tissues, and responded to repair and regeneration of the injured shell. After knockdown gene expression by injectinglncRNA7467-specific double-stranded RNA, the inner surface of the prismatic layer changed significantly, and then theN44gene was correspondingly inhibited. In addition,lncRNA7467mainly distributed in the cytoplasm, and it mays primarily play a role in post-transcriptional regulation. These works indicated thatlncRNA7467was involved in the biomineralization process in pearl oysterP. f. martensii.