High-performance liquid chromatographic analysis of cocaine in human plasma.
High-performance liquid chromatographic analysis of cocaine in human plasma.
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DOI：
10.1093/jat/4.6.305
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发表时间：
1980-11
影响因子：
2.5
通讯作者：
A. N. Masoud;D. M. Krupski
中科院分区：
文献类型：
--
作者：
A. N. Masoud;D. M. Krupski
本文首次提出了一种简单的等度HPLC法测定血浆中可卡因的方法。在乙醚中浸提碱化血浆，在乙酸中再浸提，随后在正己烷中最终浸提前碱化。将己烷浸提液蒸干，在移动的相中复溶，然后进行色谱分析。使用反相微粒C-18柱、预柱和设置在232 nm的UV检测器。采用含有75%甲醇和25% 0.05 mol/L磷酸钾缓冲液（pH 6.6）的移动的流动相，流速为0.8 mL/min。根据（a）峰高和（B）峰高与丁卡因内标物峰高的比值，测定血浆中20 - 500 ng/mL范围内的可卡因。在任一基础上，确定了浓度的线性回归。（a）和（B）的相关系数（r）分别为0.993和0.988。检查了22种常用药物的干扰情况。八种药物被认为是潜在干扰可卡因的候选药物;利多卡因和氟哌利多被发现会干扰实际患者的样本。发现仅哌替啶干扰内标物。在接受可卡因和其他药物的患者的血浆中测定了可卡因。
A simple isocratic HPLC procedure for the analysis of cocaine in plasma, with or without an internal standard, is described for the first time. Basified plasma was extracted in ether, re-extracted in acetic acid, which was subsequently basified prior to the final extraction in n-hexane. The hexane extract was evaporated to dryness, reconstituted in the mobile phase and then chromatographed. A reverse-phase micro-particulate C-18 column, a pre-column, and a UV detector set at 232 nm were used. A mobile phase containing 75% methanol and 25% 0.05 mol/L potassium phosphate buffer (pH 6.6) was used at a flow rate of 0.8 mL/min. Cocaine in the range of 20 to 500 ng/mL in plasma was determined on the basis of (a) peak height and (b) ratio of peak height to that of tetracaine internal standard. On either basis a linear regression on concentration was determined. The correlation coefficients (r) were 0.993 and 0.988 for (a) and (b) respectively. Twenty-two commonly used drugs were examined for interference. Eight drugs were considered candidates for potential interference with cocaine; lidocaine and droperidol were found to interfere in actual patients' samples. Only meperidine was found to interfere with the internal standard. Cocaine was determined in plasma from patients who received cocaine and other drugs.