Transcriptome analysis reveals the activation of neuroendocrine-immune system in shrimp hemocytes at the early stage of WSSV infection
Transcriptome analysis reveals the activation of neuroendocrine-immune system in shrimp hemocytes at the early stage of WSSV infection
复制标题
转录组分析揭示了 WSSV 感染早期虾血细胞神经内分泌免疫系统的激活
DOI：
10.1186/s12864-019-5614-4
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发表时间：
2019
期刊：
影响因子：
4.4
通讯作者：
Li Fuhua
中科院分区：
文献类型：
--
作者：
Wang Fuxuan;Li Shihao;Xiang Jianhai;Li Fuhua
背景在脊椎动物和无脊椎动物中，神经、内分泌和免疫系统之间的功能性通讯已经建立。循环血细胞作为基本球员在这个串扰，其功能是保守的主要群体的后生动物的进化。然而，在病原体infection.ResultsIn这项研究中，虾血细胞中的神经内分泌免疫（NEI）系统的作用仍然在很大程度上是未知的，我们测序6个cDNA文库制备的血细胞从凡纳滨对虾这是注射WSSV（白色斑点综合征病毒）或PBS 6小时使用Illumina Hiseq 4000平台。结果表明，WSSV感染后，血细胞中差异表达基因共3444个，其中上调基因3240个，下调基因204个。在这些基因中，349个DEG与先天免疫相关，并根据其预测功能分为七组。有趣的是，18个基因编码推定的神经肽前体显着诱导WSSV感染。此外，还将部分基因定位于NEI系统的几个典型过程，包括激素原的蛋白水解、氨基酸神经递质途径、生物胺合成和乙酰胆碱信号途径。结论WSSV感染可激活NEI系统，为进一步研究血细胞介导的NEI系统在对虾抗病毒免疫中的作用提供了新的思路。
BackgroundFunctional communications between nervous, endocrine and immune systems are well established in both vertebrates and invertebrates. Circulating hemocytes act as fundamental players in this crosstalk, whose functions are conserved during the evolution of the main groups of metazoans. However, the roles of the neuroendocrine-immune (NEI) system in shrimp hemocytes during pathogen infection remain largely unknown.ResultsIn this study, we sequenced six cDNA libraries prepared with hemocytes fromLitopenaeus vannameiwhich were injected by WSSV (white spot syndrome virus) or PBS for 6 h using Illumina Hiseq 4000 platform. As a result, 3444 differentially expressed genes (DEGs), including 3240 up-regulated genes and 204 down-regulated genes, were identified from hemocytes after WSSV infection. Among these genes, 349 DEGs were correlated with innate immunity and categorized into seven groups based on their predictive function. Interestingly, 18 genes encoded putative neuropeptide precursors were induced significantly by WSSV infection. Furthermore, some genes were mapped to several typical processes in the NEI system, including proteolytic processing of prohormones, amino acid neurotransmitter pathways, biogenic amine biosynthesis and acetylcholine signaling pathway.ConclusionsThe data suggested that WSSV infection triggers the activation of NEI in shrimp, which throws a light on the pivotal roles of NEI system mediated by hemocytes in shrimp antiviral immunity.