Genetic differences in the tail-suspension test and its relationship to imipramine response among 11 inbred strains of mice
Genetic differences in the tail-suspension test and its relationship to imipramine response among 11 inbred strains of mice
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DOI：
10.1016/s0006-3223(00)01028-3
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发表时间：
2001-04-01
影响因子：
10.6
通讯作者：
Gershenfeld, HK
中科院分区：
文献类型：
--
作者：
Liu, XQ;Gershenfeld, HK
背景：悬尾实验（TST）是一种用于检测抗抑郁药对啮齿动物行为影响的简单筛选实验。本实验研究了11种近交系小鼠在悬尾实验中不动时间所衡量的对压力情境反应的个体间差异，以及丙咪嗪（腹腔注射30mg/kg）对减少不动时间的作用。这11种近交系分别是129S6/SvEvTac、A/J、AKR/J、Balb/cJ、C3H/HeJ、C57BL/6J、DBA/2J、FVB/NJ、NMR1、SencarA/PtJ和SWR/J。
方法：所有小鼠均进行两次悬尾实验：1）自发的基础悬尾实验；2）丙咪嗪或生理盐水悬尾实验。在6分钟的测试中，不动时间是所测量的指标。
结果：在测试的4个品系中，基础悬尾实验中雌性小鼠的不动时间比雄性小鼠长。对于雄性小鼠（n = 11个品系），在基础悬尾实验和丙咪嗪反应悬尾实验中，不动时间均存在显著的品系差异，遗传力估计值分别为0.31和0.60。与生理盐水相比，丙咪嗪显著减少了DBA/2J、FVB/NJ和NMRI品系的不动时间。令人惊讶的是，丙咪嗪对不动时间的减少与基础不动时间无关。
结论：这些结果表明，基础悬尾实验的反应和丙咪嗪介导的悬尾实验反应是由不同的遗传途径介导的。《生物精神病学》2001年；49卷：575 - 581页（C）2001年生物精神病学学会。
Background: The tail suspension test (TST) is a simple screening test for the behavioral effects of antidepressants in rodents. This experiment investigated the interindividual differences in responses to stressful situations measured by duration of immobility in the TST and the effects of imipramine (30 mg/kg intraperitoneally) in reducing immobility among 11 inbred strains of mice. The 11 inbred strains were 129S6/SvEvTac, A/J, AKR/J, Balb/cJ, C3H/HeJ, C57BL/6J, DBA/2J, FVB/NJ, NMR1, SencarA/PtJ, and SWR/J.Methods: All mice underwent two trials of TST: 1) spontaneous, basal TST and 2) imipramine or saline TST. The duration of immobility was the trait measured during a 6-min test.Results: In the four strains tested, female mice had longer duration of immobility than male mice in basal TST duration of immobility. For male mice (n = 11 strains), significant strain differences in immobility duration were found for both basal TST and imipramine response TST, with heritability estimates of .31 and .60, respectively. Immobility duration for the DBA/2J, FVB/NJ, and NMRI strains were significantly reduced by imipramine, relative to saline. Surprisingly, this reduction of immobility by imipramine was independent of the basal immobility.Conclusions: These results suggest that the responses on basal TST and the imipramine-mediated responses on TST are mediated by separate genetic pathways. Biol Psychiatry 2001;49:575-581 (C) 2001 Society of Biological Psychiatry.