INHIBITION OF GAMMA-AMINOBUTYRATE AND GLYCINE UPTAKE INTO SYNAPTIC VESICLES
INHIBITION OF GAMMA-AMINOBUTYRATE AND GLYCINE UPTAKE INTO SYNAPTIC VESICLES
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DOI：
10.1016/s0922-4106(05)80040-9
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发表时间：
1991-05-25
期刊：
影响因子：
--
通讯作者：
FONNUM, F
中科院分区：
文献类型：
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作者：
CHRISTENSEN, H;FYSKE, EM;FONNUM, F
囊泡GABA和甘氨酸摄取的底物特异性进行了研究，分别从脑和脊髓囊泡组分。 甘氨酸、β-丙氨酸和γ-乙烯基-GABA是脑内突触囊泡摄取GABA的竞争性抑制剂。 同样，GABA和β-丙氨酸被证明是脊髓中甘氨酸囊泡摄取的竞争性抑制剂。 表观K（i）值与运输系统的相应K（m）值在相同范围内。 不同氨基酸的积累进行了检查，结构相关的氨基酸GABA，β-丙氨酸和甘氨酸都采取了两个囊泡馏分。 在目前的研究中，我们认为，有GABA和甘氨酸的囊泡转运蛋白的相似性，这两种氨基酸很可能采取了到相同的囊泡人口。 GABA和甘氨酸在终末的合成和高亲和力的突触体摄取可能是区分两者的关键因素。
The substrate specificity of vesicular GABA and glycine uptake was studied in vesicle fractions from brain and spinal cord, respectively. Glycine, beta-alanine and gamma-vinyl-GABA were competitive inhibitors of the GABA uptake by synaptic vesicles in brain. Likewise GABA and beta-alanine turned out to be competitive inhibitors of vesicular uptake of glycine in spinal cord. The apparent K(i) values were in the same range as the respective K(m) values for the transport systems. Accumulation of different amino acids were examined, and the structurally related amino acids GABA, beta-alanine and glycine were all taken up by both vesicle fractions. In the present study, we suggest that there are similarities in the vesicular transporters for GABA and glycine, and the two amino acids are probably taken up into the same vesicle population. The key factor in differentiating between GABA and glycine as transmitters in the terminals could be the synthesis and the high-affinity synaptosomal uptake.